ArticleRice (New York, N.Y.)2014
Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format.
Article in Rice (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Designing and testing CRISPRi-based synthetic gene circuits in plants.Nature protocols · 2026Review
- OsBRW1, a novel blast-resistant gene, coded a NBS-LRR protein to interact with OsSRFP1 to balance rice growth and resistance.Plant biotechnology journal · 2025Article
- Split MutT prevents the mutator phenotype of mutT-deficient Escherichia coli.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2024Article
- Establishing an efficient protoplast transient expression system for investigation of floral thermogenesis in aroids.Plant cell reports · 2022Article
- Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress.Frontiers in plant science · 2022Review
- Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc.Plant biotechnology (Tokyo, Japan) · 2021Article
- Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions.Plants (Basel, Switzerland) · 2020Review
- Root angle modifications by theProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Soft mechanical stimulation induces a defense response against Botrytis cinerea in strawberry.Plant cell reports · 2018Article
- Development of a rapid, low-cost protoplast transfection system for switchgrass (Panicum virgatum L.).Plant cell reports · 2016Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe rice interactome, in which a network of protein-protein interactions has been elucidated in rice, is a useful resource to identify functional modules of rice signal transduction pathways. Protein-protein interactions occur in cells in two ways, constitutive and regulative. While a yeast-based high-throughput method has been widely used to identify the constitutive interactions, a method to detect the regulated interactions is rarely developed for a large-scale analysis.
resultsA split luciferase complementation assay was applied to detect the regulated interactions in rice. A transformation method of rice protoplasts in a 96-well plate was first established for a large-scale analysis. In addition, an antibody that specifically recognizes a carboxyl-terminal fragment of Renilla luciferase was newly developed. A pair of antibodies that recognize amino- and carboxyl- terminal fragments of Renilla luciferase, respectively, was then used to monitor quality and quantity of interacting recombinant-proteins accumulated in the cells. For a proof-of-concept, the method was applied to detect the gibberellin-dependent interaction between GIBBERELLIN INSENSITIVE DWARF1 and SLENDER RICE 1.
conclusionsA method to detect regulated protein-protein interactions was developed towards establishment of the rice interactome.
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