ArticleMolecular & cellular proteomics : MCP2014
Quantitative proteomic discovery of dynamic epigenome changes that control human cytomegalovirus (HCMV) infection.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Review
- Neuronal Dot1l Activity Acts as a Mitochondrial Gene-Repressor Associated with Human Brain Aging via H3K79 Hypermethylation.International journal of molecular sciences · 2023Article
- Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection.Virologica Sinica · 2022Article
- NADFrontiers in molecular biosciences · 2021Review
- Article
- Targeting the Histone Methyltransferase Disruptor of Telomeric Silencing 1-Like Restricts Avian Leukosis Virus Subgroup J Replication by Restoring the Innate Immune Response in Chicken Macrophages.Frontiers in microbiology · 2020Article
- Epigenetic control of influenza virus: role of H3K79 methylation in interferon-induced antiviral response.Scientific reports · 2018Article
- Cellular responses to human cytomegalovirus infection: Induction of a mesenchymal-to-epithelial transition (MET) phenotype.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Promise of Proteomics in the Study of Oncogenic Viruses.Molecular & cellular proteomics : MCP · 2017Review
- Plasma L-Carnitine and L-Lysine Concentrations in HIV-Infected Patients.The open biochemistry journal · 2017Article
- Determining the Mitochondrial Methyl Proteome in Saccharomyces cerevisiae using Heavy Methyl SILAC.Journal of proteome research · 2016Article
- The therapeutic potential of epigenetic manipulation during infectious diseases.Pharmacology & therapeutics · 2016Review
- The upstreams and downstreams of H3K79 methylation by DOT1L.Chromosoma · 2016Review
- Intricate Roles of Mammalian Sirtuins in Defense against Viral Pathogens.Journal of virology · 2016Review
- Characterization of histone post-translational modifications during virus infection using mass spectrometry-based proteomics.Methods (San Diego, Calif.) · 2015Article
- The life cycle and pathogenesis of human cytomegalovirus infection: lessons from proteomics.Expert review of proteomics · 2014Article
- Manipulation of host pathways by human cytomegalovirus: insights from genome-wide studies.Seminars in immunopathology · 2014Review
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
This work represents the first comprehensive quantitative analysis of global histone post-translational modifications (PTMs) from a virus infection, namely human cytomegalovirus (HCMV) infection. We used a nanoLC-MS/MS platform to identify and quantify the dynamic histone H3 and H4 PTMs expressed during HCMV replication in primary fibroblasts. Specifically, we examined the changes in histone PTMs over a 96 h time course to sample the immediate early (IE), early (E), and late (L) stages of viral infection. Several changes in histone H3 and H4 PTMs were observed, including a marked increase in H3K79me2 and H3K27me3K36me2, and a decrease in H4K16ac, highlighting likely epigenetic strategies of transcriptional activation and silencing during HCMV lytic infection. Heavy methyl-SILAC (hm-SILAC) was used to further confirm the histone methylation flux (especially for H3K79) during HCMV infection. We evaluated DOT1L (the H3K79 methyltransferase) mRNA levels in mock and HCMV-infected cells over a 96 h time course, and observed a significant increase in this methyltransferase as early as 24 hpi showing that viral infection up-regulates DOT1L expression, which drives H3K79me2. We then used shRNA to create a DOT1L knockdown cell population, and found that HCMV infection of the knockdown cells resulted in a 10-fold growth defect when compared with infected control cells not subjected to knockdown. This work documents multiple histone PTMs that occur in response to HCMV infection of fibroblasts, and provides a framework for evaluation of the role of epigenetic modifications in the virus-host interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.