ArticleJournal of experimental botany2014
Dynamic changes in the subcellular distribution of the tobacco ROS-producing enzyme RBOHD in response to the oomycete elicitor cryptogein.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- SOBIR1 integrates pathogen recognition and hormonal metabolism in Nicotiana tabacum.Plant physiology · 2026Article
- Reactive oxygen species and oxidative signalling in plants.Essays in biochemistry · 2026Review
- The engineered Immunoprotein enhance broad-spectrum disease resistance in solanaceous crops.BMC plant biology · 2026Article
- FLS2-RBOHD module regulates changes in the metabolome ofPlant-environment interactions (Hoboken, N.J.) · 2023Article
- Transcriptome analysis of sugarcane reveals rapid defense response of SES208 to Xanthomonas albilineans in early infection.BMC plant biology · 2023Article
- Characteristics, Roles and Applications of Proteinaceous Elicitors from Pathogens in Plant Immunity.Life (Basel, Switzerland) · 2023Review
- Genome-wide analysis of respiratory burst oxidase homolog gene family in pea (Frontiers in plant science · 2023Article
- Endoplasmic Reticulum Stress and Reactive Oxygen Species in Plants.Antioxidants (Basel, Switzerland) · 2022Review
- FLS2-RBOHD-PIF4 Module Regulates Plant Response to Drought and Salt Stress.International journal of molecular sciences · 2022Article
- Membrane nanodomains and transport functions in plant.Plant physiology · 2021Review
- Focal Accumulation of ROS Can BlockInternational journal of molecular sciences · 2020Article
- Plant Cells under Attack: Unconventional Endomembrane Trafficking during Plant Defense.Plants (Basel, Switzerland) · 2020Review
- Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions.Frontiers in genetics · 2020Review
- The Respiratory Burst Oxidase Homolog D (RbohD) Cell and Tissue Distribution in Potato-Potato Virus Y (PVYInternational journal of molecular sciences · 2019Article
- On the Origin and Fate of Reactive Oxygen Species in Plant Cell Compartments.Antioxidants (Basel, Switzerland) · 2019Review
- Aphid-borne Viral Spread Is Enhanced by Virus-induced Accumulation of Plant Reactive Oxygen Species.Plant physiology · 2019Article
- Generation of Superoxide by OeRbohH, a NADPH Oxidase Activity During Olive (Frontiers in plant science · 2019Article
- Interactions between lipids and proteins are critical for organization of plasma membrane-ordered domains in tobacco BY-2 cells.Journal of experimental botany · 2018Article
- Reactive Oxygen Species and Gibberellin Acid Mutual Induction to Regulate Tobacco Seed Germination.Frontiers in plant science · 2018Article
- Brassinosteroids regulate root growth by controlling reactive oxygen species homeostasis and dual effect on ethylene synthesis in Arabidopsis.PLoS genetics · 2018Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant NADPH oxidases, also known as respiratory burst oxidase homologues (RBOHs), have been identified as a major source of reactive oxygen species (ROS) during plant-microbe interactions. The subcellular localization of the tobacco (Nicotiana tabacum) ROS-producing enzyme RBOHD was examined in Bright Yellow-2 cells before and after elicitation with the oomycete protein cryptogein using electron and confocal microscopy. The plasma membrane (PM) localization of RBOHD was confirmed and immuno-electron microscopy on purified PM vesicles revealed its distribution in clusters. The presence of the protein fused to GFP was also seen in intracellular compartments, mainly Golgi cisternae. Cryptogein induced, within 1h, a 1.5-fold increase in RBOHD abundance at the PM and a concomitant decrease in the internal compartments. Use of cycloheximide revealed that most of the proteins targeted to the PM upon elicitation were not newly synthesized but may originate from the Golgi pool. ROS accumulation preceded RBOHD transcript- and protein-upregulation, indicating that ROS resulted from the activation of a PM-resident pool of enzymes, and that enzymes newly addressed to the PM were inactive. Taken together, the results indicate that control of RBOH abundance and subcellular localization may play a fundamental role in the mechanism of ROS production.
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