Evidence map›Paper›PMID 24984963›Full record

ArticleCurrent protocols in cytometry2014

The application of KillerRed for acute protein inactivation in living cells.

Timothy S Jarvela, Adam D Linstedt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols in cytometry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Molecular biology of the cell · 2021
    Article
  2. Light-Activated ROS Production Induces Synaptic Autophagy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Timothy S JarvelaDepartment of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania.
Adam D Linstedt

Funding

RETRIEVAL PATHWAY IN GOLGI STACK TARGETINGR01GM056779 · NIGMS · CARNEGIE-MELLON UNIVERSITY · PI LINSTEDT, ADAM D · 1998 to 2015
$4.1M
STRUCTURAL BASIS OF ORGANELLE TETHERINGR01GM095549 · NIGMS · CARNEGIE-MELLON UNIVERSITY · PI LINSTEDT, ADAM D · 2011 to 2014
$1.2M
NIGMS NIH HHS GM0811101NIGMS NIH HHS GM095549NIGMS NIH HHS GM56779NIGMS NIH HHS R01 GM056779NIGMS NIH HHS R01 GM095549
6 · The paper itself

Abstract

Generating loss of protein function is a powerful investigatory tool particularly if carried out on a physiologically relevant timescale in a live-cell fluorescent imaging experiment. KillerRed mediated chromophore assisted light inactivation (CALI) uses genetic encoding for specificity and light for acute inactivation that can also be spatially restricted. This unit provides protocols for setting up and carrying out properly controlled KillerRed experiments during live-cell imaging of cultured cells.

Indexed as

AnimalsCell LineGreen Fluorescent ProteinsHumansMicroscopy, FluorescenceRecombinant Fusion ProteinsGreen Fluorescent Proteinskiller red protein, AnthomedusaeRecombinant Fusion Proteinsacute inactivationCALIKillerRedprotein inactivationROS

Identifiers

PMID24984963
PMCPMC5333992

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.