ArticlePloS one2014
Bioluminescence imaging of Chlamydia muridarum ascending infection in mice.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
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- Diverse animal models forFrontiers in microbiology · 2024Review
- Reemergence of the Murine Bacterial PathogenComparative medicine · 2022Article
- Bringing genetics to heretofore intractable obligate intracellular bacterial pathogens: Chlamydia and beyond.PLoS pathogens · 2022Article
- Epitope-Based Vaccines against theVaccines · 2022Article
- Characterization of Pathogenic CD8Infection and immunity · 2022Article
- The hormonal environment and estrogen receptor signaling altersFrontiers in cellular and infection microbiology · 2022Article
- Inter-species lateral gene transfer focused on the Chlamydia plasticity zone identifies loci associated with immediate cytotoxicity and inclusion stability.Molecular microbiology · 2021Article
- Gastrointestinal Chlamydia-Induced CD8Infection and immunity · 2021Article
- Article
- Adoptive Transfer of Group 3-Like Innate Lymphoid Cells Restores Mouse Colon Resistance to Colonization of a Gamma Interferon-Susceptible Chlamydia muridarum Mutant.Infection and immunity · 2021Article
- Effects of Immunomodulatory Drug Fingolimod (FTY720) onInfection and immunity · 2020Article
- Gastrointestinal Coinfection Promotes Chlamydial Pathogenicity in the Genital Tract.Infection and immunity · 2020Article
- Evasion of Innate Lymphoid Cell-Regulated Gamma Interferon Responses by Chlamydia muridarum To Achieve Long-Lasting Colonization in Mouse Colon.Infection and immunity · 2020Article
- Article
- The Structural Integrity of Plasmid-Encoded Pgp3 Is Essential for Induction of Hydrosalpinx byFrontiers in cellular and infection microbiology · 2019Article
- Chlamydia Spreading from the Genital Tract to the Gastrointestinal Tract - A Two-Hit Hypothesis.Trends in microbiology · 2018Review
- The Genital Tract Virulence Factor pGP3 Is Essential for Chlamydia muridarum Colonization in the Gastrointestinal Tract.Infection and immunity · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Chlamydial pathogenicity in the upper genital tract relies on chlamydial ascending from the lower genital tract. To monitor chlamydial ascension, we engineered a luciferase-expressing C. muridarum. In cells infected with the luciferase-expressing C. muridarum, luciferase gene expression and enzymatic activity (measured as bioluminescence intensity) correlated well along the infection course, suggesting that bioluminescence can be used for monitoring chlamydial replication. Following an intravaginal inoculation with the luciferase-expressing C. muridarum, 8 of 10 mice displayed bioluminescence signal in the lower with 4 also in the upper genital tracts on day 3 after infection. By day 7, all 10 mice developed bioluminescence signal in the upper genital tracts. The bioluminescence signal was maintained in the upper genital tract in 6 and 2 mice by days 14 and 21, respectively. The bioluminescence signal was no longer detectable in any of the mice by day 28. The whole body imaging approach also revealed an unexpected airway infection following the intravaginal inoculation. Although the concomitant airway infection was transient and did not significantly alter the genital tract infection time courses, caution should be taken during data interpretation. The above observations have demonstrated that C. muridarum can not only achieve rapid ascending infection in the genital tract but also cause airway infection following a genital tract inoculation. These findings have laid a foundation for further optimizing the C. muridarum intravaginal infection murine model for understanding chlamydial pathogenic mechanisms.
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