ArticleThe Journal of cell biology2014
Accurate phosphoregulation of kinetochore-microtubule affinity requires unconstrained molecular interactions.
Article in The Journal of cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 114 citations in OpenAlex.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Low kinetochore-microtubule occupancy leaves a mitotic memory by delaying checkpoint silencing.Nature communications · 2026Article
- Developmental regulation of kinetochore phosphorylation determines mitotic fidelity.bioRxiv : the preprint server for biology · 2026Article
- Microtubules guide Aurora B substrate geometries for accurate chromosome segregation.Science advances · 2026Article
- Ndc80 complex, a conserved coupler for kinetochore-microtubule motility, is a sliding molecular clutch.Science advances · 2025Article
- AURKA controls oocyte spindle assembly checkpoint and chromosome alignment by HEC1 phosphorylation.Life science alliance · 2025Article
- SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.Current biology : CB · 2025Article
- CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation.Life science alliance · 2025Article
- α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments.Nature communications · 2024Article
- SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.bioRxiv : the preprint server for biology · 2024Article
- Measuring and modeling the dynamics of mitotic error correction.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Higher-order protein assembly controls kinetochore formation.Nature cell biology · 2024Article
- Principles and dynamics of spindle assembly checkpoint signalling.Nature reviews. Molecular cell biology · 2023Review
- The RPA-RNF20-SNF2H cascade promotes proper chromosome segregation and homologous recombination repair.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Strain stiffening of Ndc80 complexes attached to microtubule plus ends.Biophysical journal · 2022Article
- Hyper-active RAS/MAPK introduces cancer-specific mitotic vulnerabilities.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Small changes in phospho-occupancy at the kinetochore-microtubule interface drive mitotic fidelity.The Journal of cell biology · 2022Article
- The Astrin-SKAP complex reduces friction at the kinetochore-microtubule interface.Current biology : CB · 2022Article
- The Role of Mitotic Kinases and the RZZ Complex in Kinetochore-Microtubule Attachments: Doing the Right Link.Frontiers in cell and developmental biology · 2022Review
- The Aurora B gradient sustains kinetochore stability in anaphase.Cell reports · 2021Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Accurate chromosome segregation relies on dynamic interactions between microtubules (MTs) and the NDC80 complex, a major kinetochore MT-binding component. Phosphorylation at multiple residues of its Hec1 subunit may tune kinetochore-MT binding affinity for diverse mitotic functions, but molecular details of such phosphoregulation remain elusive. Using quantitative analyses of mitotic progression in mammalian cells, we show that Hec1 phosphorylation provides graded control of kinetochore-MT affinity. In contrast, modeling the kinetochore interface with repetitive MT binding sites predicts a switchlike response. To reconcile these findings, we hypothesize that interactions between NDC80 complexes and MTs are not constrained, i.e., the NDC80 complexes can alternate their binding between adjacent kinetochore MTs. Experiments using cells with phosphomimetic Hec1 mutants corroborate predictions of such a model but not of the repetitive sites model. We propose that accurate regulation of kinetochore-MT affinity is driven by incremental phosphorylation of an NDC80 molecular "lawn," in which the NDC80-MT bonds reorganize dynamically in response to the number and stability of MT attachments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.