ArticleProceedings of the National Academy of Sciences of the United States of America2014
Crystal structure of the photosensing module from a red/far-red light-absorbing plant phytochrome.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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110 citing papers in PubMed, 223 citations in OpenAlex.
- Hypsochromic shift in phytochrome C Pr absorption complements phytochrome B-mediated inhibition of hypocotyl elongation.The Plant journal : for cell and molecular biology · 2026Article
- Twenty-five years of photobodies: formation, composition, and the 2-compartment logic of phytochrome B signaling.Plant physiology · 2026Review
- Phytochrome B sets condensate number through graded nucleator states and seeding-site efficacy.Nature communications · 2026Article
- Intragenic suppressor screen of YHB identifies novel and known loss-of-function alleles of Arabidopsis phytochrome B.bioRxiv : the preprint server for biology · 2026Article
- Characterizing Phytochrome Photochemistry Using UV-Visible Spectroscopy in Host Cell Extracts and Purified Protein.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Circular dichroism spectroscopy reveals multiple phytochrome photoproducts in equilibrium.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- Pr and Pfr structures of plant phytochrome A.Nature communications · 2025Article
- A multisensor high-temperature signaling framework for triggering daytime thermomorphogenesis in Arabidopsis.Nature communications · 2025Article
- Structural insight into PIF6-mediated red light signal transduction of plant phytochrome B.Cell discovery · 2025Article
- Structures of myxobacterial phytochrome revealed by cryo-EM using the Spotiton technique and with x-ray crystallography.Structural dynamics (Melville, N.Y.) · 2025Article
- Both phytochrome A and phyB interact with PHYTOCHROME-INTERACTING FACTORs through an evolutionary conserved phyNature communications · 2025Article
- Phycocyanobilin Binding and Specific Amino Acid Residues Near The Chromophore Contribute To Orange Light Perception By The Dualchrome Phytochrome Region.Plant & cell physiology · 2025Article
- Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.IUCrJ · 2025Article
- Plant Phytochrome Interactions Decode Light and Temperature Signals.The Plant cell · 2024Article
- Modulation of warm temperature-sensitive growth using a phytochrome B dark reversion variant, phyB[G515E], in Arabidopsis and rice.Journal of advanced research · 2024Article
- Signaling by a bacterial phytochrome histidine kinase involves a conformational cascade reorganizing the dimeric photoreceptor.Nature communications · 2024Article
- Photoreception and signaling in bacterial phytochrome revealed by single-particle cryo-EM.Science advances · 2024Article
- The role of red and white light in optimizing growth and accumulation of plant specialized metabolites at two light intensities in medical cannabis (Frontiers in plant science · 2024Article
- Responding to light signals: a comprehensive update on photomorphogenesis in cyanobacteria.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Plant phytochrome A in the Pr state assembles as an asymmetric dimer.Cell research · 2023Article
50 more citing papers are in PubMed but not listed here.
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Many aspects of plant photomorphogenesis are controlled by the phytochrome (Phy) family of bilin-containing photoreceptors that detect red and far-red light by photointerconversion between a dark-adapted Pr state and a photoactivated Pfr state. Whereas 3D models of prokaryotic Phys are available, models of their plant counterparts have remained elusive. Here, we present the crystal structure of the photosensing module (PSM) from a seed plant Phy in the Pr state using the PhyB isoform from Arabidopsis thaliana. The PhyB PSM crystallized as a head-to-head dimer with strong structural homology to its bacterial relatives, including a 5(Z)syn, 10(Z)syn, 15(Z)anti configuration of the phytochromobilin chromophore buried within the cGMP phosphodiesterase/adenylyl cyclase/FhlA (GAF) domain, and a well-ordered hairpin protruding from the Phy-specific domain toward the bilin pocket. However, its Per/Arnt/Sim (PAS) domain, knot region, and helical spine show distinct structural differences potentially important to signaling. Included is an elongated helical spine, an extended β-sheet connecting the GAF domain and hairpin stem, and unique interactions between the region upstream of the PAS domain knot and the bilin A and B pyrrole rings. Comparisons of this structure with those from bacterial Phys combined with mutagenic studies support a toggle model for photoconversion that engages multiple features within the PSM to stabilize the Pr and Pfr end states after rotation of the D pyrrole ring. Taken together, this Arabidopsis PhyB structure should enable molecular insights into plant Phy signaling and provide an essential scaffold to redesign their activities for agricultural benefit and as optogenetic reagents.
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