Evidence map›Paper›PMID 24981863›Full record

ArticleCell reports2014

Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites.

Schraga Schwartz, Maxwell R Mumbach, Marko Jovanovic, Tim Wang, Karolina Maciag, G Guy Bushkin, Philipp Mertins, Dmitry Ter-Ovanesyan, Naomi Habib, Davide Cacchiarelli and 10 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 840 papers.

0numbers the graph read from it
0cells of the map it votes in
840citing papers in PubMed
21.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

840 citing papers in PubMed, 1,241 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. The role of mNaunyn-Schmiedeberg's archives of pharmacology · 2026
    Review
  7. mNature reviews. Neuroscience · 2026
    Review
  8. Review
  9. Review
  10. Review
  11. WTAP tetramer ensures mEMBO reports · 2026
    Article
  12. Review
  13. Article
  14. Review
  15. Methylation marks breast cancer metastasis: The roles of mThe Journal of biological chemistry · 2026
    Review
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article

780 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 5 institutions in 1 country.

Schraga SchwartzBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Maxwell R MumbachBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Marko JovanovicBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Tim WangBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Karolina MaciagBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Graduate Program in Immunology, Division of Medical Sciences, Harvard Medical School, Boston, MA 02115, USA.
G Guy BushkinWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Philipp MertinsBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Dmitry Ter-OvanesyanBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Naomi HabibBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Davide CacchiarelliBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Stem Cell Institute and Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Neville E SanjanaBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Elizaveta FreinkmanWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Michael E PacoldWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA; Department of Radiation Oncology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, MA 02215, USA.
Rahul SatijaBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Tarjei S MikkelsenBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Stem Cell Institute and Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Nir HacohenBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Charlestown, MA 02129, USA.
Feng ZhangBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Brain and Cognitive Sciences and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Steven A CarrBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Eric S LanderBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Systems Biology, Harvard Medical School, Boston, MA 02114, USA. Electronic address: lander@broadinstitute.org.
Aviv RegevBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815, USA. Electronic address: aregev@broad.mit.edu.
Broad Institute · USWhitehead Institute for Biomedical Research · USHarvard Stem Cell Institute · USMassachusetts General Hospital · USMassachusetts Institute of Technology · US

Funding

Large Scale Sequencing and Analysis of GenomesU54HG003067 · NHGRI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI GABRIEL, STACEY, LANDER, ERIC S · 2004 to 2015
$568.6M
VIRUS PRODUCTION COREP30CA014051 · NCI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI Andreea O'Connell · 1985 to 2026
$93.9M
Medical Scientist Training ProgramT32GM007753 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI WALENSKY, LOREN DAVID · 1985 to 2021
$50.0M
PRE-DOCTORAL TRAINING IN THE BIOLOGICAL SCIENCEST32GM007287 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BELL, STEPHEN P., KEATING, AMY E · 1985 to 2019
$40.8M
Training in Molecular, Cellular, and Chemical BiologyT32GM007598 · NIGMS · HARVARD UNIVERSITY · PI DULAC, CATHERINE · 1985 to 2019
$16.2M
Center for Cell CircuitsP50HG006193 · NHGRI · BROAD INSTITUTE, INC. · PI REGEV, AVIV · 2011 to 2015
$15.6M
Probing Neuropsychiatric Diseases Using Targeted Epigenome and Genome EngineeringDP1MH100706 · NIMH · BROAD INSTITUTE, INC. · PI ZHANG, FENG · 2012 to 2016
$4.3M
The temporal reconfiguration of regulatory networks: From yeast to cancerDP1CA174427 · NCI · BROAD INSTITUTE, INC. · PI REGEV, AVIV · 2012 to 2012
$839k
Howard Hughes Medical InstituteNCI NIH HHS DP1 CA174427NCI NIH HHS P30 CA014051NHGRI NIH HHS P50 HG006193NHGRI NIH HHS U54 HG003067NIGMS NIH HHS T32 GM007287NIGMS NIH HHS T32 GM007598NIGMS NIH HHS T32 GM007753NIMH NIH HHS DP1 MH100706
6 · The paper itself

Abstract

N6-methyladenosine (m6A) is a common modification of mRNA with potential roles in fine-tuning the RNA life cycle. Here, we identify a dense network of proteins interacting with METTL3, a component of the methyltransferase complex, and show that three of them (WTAP, METTL14, and KIAA1429) are required for methylation. Monitoring m6A levels upon WTAP depletion allowed the definition of accurate and near single-nucleotide resolution methylation maps and their classification into WTAP-dependent and -independent sites. WTAP-dependent sites are located at internal positions in transcripts, topologically static across a variety of systems we surveyed, and inversely correlated with mRNA stability, consistent with a role in establishing "basal" degradation rates. WTAP-independent sites form at the first transcribed base as part of the cap structure and are present at thousands of sites, forming a previously unappreciated layer of transcriptome complexity. Our data shed light on the proteomic and transcriptional underpinnings of this RNA modification.

Indexed as

5' Untranslated RegionsRNA Processing, Post-TranscriptionalAnimalsHEK293 CellsHumansMethylationMethyltransferasesMiceRNA, MessengerRNA Stability5' Untranslated Regions6-methyladenine mRNA methyltransferaseMethyltransferasesRNA, Messenger

Identifiers

PMID24981863
PMCPMC4142486
OpenAlexW2102872651

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.