ArticleThe Journal of general physiology2014
Calcium-calmodulin does not alter the anion permeability of the mouse TMEM16A calcium-activated chloride channel.
Article in The Journal of general physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 37 citations in OpenAlex.
- TMEM16A channel signalling microdomains in the regulation of vascular function.The Journal of physiology · 2026Review
- Structure and Function of Calcium-Activated Chloride Channels and Phospholipid Scramblases in the TMEM16 Family.Handbook of experimental pharmacology · 2024Review
- Molecular mechanisms of activation and regulation of ANO1-Encoded CaChannels (Austin, Tex.) · 2021Review
- Divalent Cation Modulation of Ion Permeation in TMEM16 Proteins.International journal of molecular sciences · 2021Article
- Bicarbonate permeation through anion channels: its role in health and disease.Pflugers Archiv : European journal of physiology · 2020Review
- Regulation of CFTR Bicarbonate Channel Activity by WNK1: Implications for Pancreatitis and CFTR-Related Disorders.Cellular and molecular gastroenterology and hepatology · 2020Article
- Cobalt ion interaction with TMEM16A calcium-activated chloride channel: Inhibition and potentiation.PloS one · 2020Article
- NaFrontiers in physiology · 2020Article
- Molecular basis of PIPNature communications · 2019Article
- Dynamic change of electrostatic field in TMEM16F permeation pathway shifts its ion selectivity.eLife · 2019Article
- Comparison of ion transport determinants between a TMEM16 chloride channel and phospholipid scramblase.The Journal of general physiology · 2019Article
- Known structures and unknown mechanisms of TMEM16 scramblases and channels.The Journal of general physiology · 2018Review
- Calcium-Activated ClInternational journal of molecular sciences · 2018Review
- Bioinformatic characterization of the Anoctamin Superfamily of Ca2+-activated ion channels and lipid scramblases.PloS one · 2018Article
- Independent activation of distinct pores in dimeric TMEM16A channels.The Journal of general physiology · 2016Article
- HCO3- Transport through Anoctamin/Transmembrane Protein ANO1/TMEM16A in Pancreatic Acinar Cells Regulates Luminal pH.The Journal of biological chemistry · 2016Article
- Influence of intracellular Ca2+ and alternative splicing on the pharmacological profile of ANO1 channels.American journal of physiology. Cell physiology · 2016Article
- Pore dilatation increases the bicarbonate permeability of CFTR, ANO1 and glycine receptor anion channels.The Journal of physiology · 2016Article
- Calmodulin regulation of TMEM16A and 16B Ca(2+)-activated chloride channels.Channels (Austin, Tex.) · 2016Review
- PKCα regulates TMEM16A-mediated Cl⁻ secretion in human biliary cells.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
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Abstract
The transmembrane protein TMEM16A forms a Ca(2+)-activated Cl(-) channel that is permeable to many anions, including SCN(-), I(-), Br(-), Cl(-), and HCO3 (-), and has been implicated in various physiological functions. Indeed, controlling anion permeation through the TMEM16A channel pore may be critical in regulating the pH of exocrine fluids such as the pancreatic juice. The anion permeability of the TMEM16A channel pore has recently been reported to be modulated by Ca(2+)-calmodulin (CaCaM), such that the pore of the CaCaM-bound channel shows a reduced ability to discriminate between anions as measured by a shift of the reversal potential under bi-ionic conditions. Here, using a mouse TMEM16A clone that contains the two previously identified putative CaM-binding motifs, we were unable to demonstrate such CaCaM-dependent changes in the bi-ionic potential. We confirmed the activity of CaCaM used in our study by showing CaCaM modulation of the olfactory cyclic nucleotide-gated channel. We suspect that the different bi-ionic potentials that were obtained previously from whole-cell recordings in low and high intracellular [Ca(2+)] may result from different degrees of bi-ionic potential shift secondary to a series resistance problem, an ion accumulation effect, or both.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.