ArticleInfection and immunity2014
Two functional type VI secretion systems in avian pathogenic Escherichia coli are involved in different pathogenic pathways.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers.
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46 citing papers in PubMed, 79 citations in OpenAlex.
- The (p)ppGpp Synthetase RelA Contributes to Virulence, Competition Capability and Antibiotic Resistance of Avian PathogenicMicroorganisms · 2026Article
- Neisseria subflava Type 6 Secretion System competition with bacterial and fungal species.Applied microbiology and biotechnology · 2026Article
- Effects of the type V secretion system eYadA on the biological properties and pathogenicity of avian pathogenic Escherichia coli.Veterinary research · 2026Article
- Type VI secretion systems at the interface between bacteria and eukaryotic cells.Genetics and molecular biology · 2026Article
- Predicting clinical outcome ofMicrobial genomics · 2025Article
- NhaA facilitates the maintenance of bacterial envelope integrity and the evasion of complement attack contributing to extraintestinal pathogenicInfection and immunity · 2023Article
- Parallel loss of type VI secretion systems in two multi-drug-resistantMicrobial genomics · 2023Article
- Antimicrobial Efficacy of Cinnamon Essential Oil against Avian PathogenicAnimals : an open access journal from MDPI · 2023Article
- Diversity of the type VI secretion systems in theMicrobial genomics · 2023Article
- Effector protein Hcp2a of avian pathogenic Escherichia coli interacts with the endoplasmatic reticulum associated RPL23 protein of chicken DF-1 fibroblasts.Veterinary research · 2023Article
- Activity and Crystal Structure of the Adherent-InvasiveInternational journal of molecular sciences · 2023Article
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- Melatonin Is Neuroprotective inInternational journal of molecular sciences · 2022Article
- RyhB in Avian PathogenicInternational journal of molecular sciences · 2022Article
- The Role ofFoods (Basel, Switzerland) · 2022Article
- Hcp Proteins of the Type VI Secretion System Promote Avian PathogenicLife (Basel, Switzerland) · 2022Article
- Whole-genome sequencing analysis of Shiga toxin-producing Escherichia coli O22:H8 isolated from cattle prediction pathogenesis and colonization factors and position in STEC universe phylogeny.Journal of microbiology (Seoul, Korea) · 2022Article
- Phosphopantetheinyl transferase ClbA contributes to the virulence of avian pathogenic Escherichia coli in meningitis infection of mice.PloS one · 2022Article
- Fur functions as an activator of T6SS-mediated bacterial dominance and virulence inFrontiers in microbiology · 2022Article
- Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenicFrontiers in microbiology · 2022Review
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type VI secretion systems (T6SSs) are involved in the pathogenicity of several Gram-negative bacteria. The VgrG protein, a core component and effector of T6SS, has been demonstrated to perform diverse functions. The N-terminal domain of VgrG protein is a homologue of tail fiber protein gp27 of phage T4, which performs a receptor binding function and determines the host specificity. Based on sequence analysis, we found that two putative T6SS loci exist in the genome of the avian pathogenic Escherichia coli (APEC) strain TW-XM. To assess the contribution of these two T6SSs to TW-XM pathogenesis, the crucial clpV clusters of these two T6SS loci and their vgrG genes were deleted to generate a series of mutants. Consequently, T6SS1-associated mutants presented diminished adherence to and invasion of several host cell lines cultured in vitro, decreased pathogenicity in duck and mouse infection models in vivo, and decreased biofilm formation and bacterial competitive advantage. In contrast, T6SS2-associated mutants presented a significant decrease only in the adherence to and invasion of mouse brain microvascular endothelial cell (BMEC) line bEnd.3 and brain tissue of the duck infection model. These results suggested that T6SS1 was involved in the proliferation of APEC in systemic infection, whereas VgrG-T6SS2 was responsible only for cerebral infection. Further study demonstrated that VgrG-T6SS2 was able to bind to the surface of bEnd.3 cells, whereas it did not bind to DF-1 (chicken embryo fibroblast) cells, which further proved the interaction of VgrG-T6SS2 with the surface of BMECs.
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