ArticleTissue engineering. Part A2015
Decellularized tracheal extracellular matrix supports epithelial migration, differentiation, and function.
Article in Tissue engineering. Part A, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 46 citations in OpenAlex.
- Cryopreservation preserves the morpho-structural and mechanical integrity of human decellularized tracheas and supports a reduced immunogenic profile.Frontiers in bioengineering and biotechnology · 2026Article
- Matrix-free human lung organoids derived from induced pluripotent stem cells to model lung injury.Stem cell research & therapy · 2024Article
- Regeneration Process of an Autologous Tissue-Engineered Trachea (aTET) in a Rat Patch Tracheoplasty Model.Bioengineering (Basel, Switzerland) · 2024Article
- Partial decellularization eliminates immunogenicity in tracheal allografts.Bioengineering & translational medicine · 2023Article
- Article
- Tracheal Macrophages During Regeneration and Repair of Long-Segment Airway Defects.The Laryngoscope · 2022Article
- Article
- Homogeneous Distribution of Exogenous Cells onto De-epithelialized Rat Trachea via Instillation of Cell-Loaded Hydrogel.ACS biomaterials science & engineering · 2022Article
- Perpendicular implantation of porcine trachea extracellular matrix for enhanced xenogeneic scaffold surface epithelialization in a canine model.Frontiers in surgery · 2022Article
- Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal.Bioactive materials · 2021Review
- Human Bronchial Epithelial Cell Growth on Homologous Versus Heterologous Tissue Extracellular Matrix.The Journal of surgical research · 2021Article
- Fabrication of Decellularized Engineered Extracellular Matrix through Bioreactor-Based Environment for Bone Tissue Engineering.ACS omega · 2020Article
- Long-term observation of airway reconstruction using decellularized tracheal allografts in micro-miniature pigs at growing stage.Regenerative therapy · 2020Article
- Tissue engineering applications in otolaryngology-The state of translation.Laryngoscope investigative otolaryngology · 2020Review
- Bioengineered airway epithelial grafts with mucociliary function based on collagen IV- and laminin-containing extracellular matrix scaffolds.The European respiratory journal · 2020Article
- Deconstructing tissue engineered trachea: Assessing the role of synthetic scaffolds, segmental replacement and cell seeding on graft performance.Acta biomaterialia · 2020Article
- Current and Alternative Therapies for Nasal Mucosa Injury: A Review.International journal of molecular sciences · 2020Review
- Factors Influencing Poor Outcomes in Synthetic Tissue-Engineered Tracheal Replacement.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2019Article
- Mouse Model of Tracheal Replacement With Electrospun Nanofiber Scaffolds.The Annals of otology, rhinology, and laryngology · 2019Article
- A Comparative Study of the Effects of Different Decellularization Methods and Genipin-Cross-Linking on the Properties of Tracheal Matrices.Tissue engineering and regenerative medicine · 2019Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Tracheal loss is a source of significant morbidity for affected patients with no acceptable solution. Interest in engineering tracheal transplants has created a demand for small animal models of orthotopic tracheal transplantation. Here, we examine the use of a decellularized graft in a murine model of tracheal replacement. Fresh or decellularized tracheas harvested from age-matched female donor C57BL/6 mice were transplanted into syngeneic recipients. Tracheas were decellularized using repeated washes of water, 3% Triton X-100, and 3 M NaCl under cyclic pressure changes, followed by disinfection with 0.1% peracetic acid/4% ethanol, and terminal sterilization by gamma irradiation. Tracheas were explanted for immunolabeling at 1, 4, and 8 weeks following surgery. Video microscopy and computed tomography were performed to assess function and structure. Decellularized grafts supported complete reepithelialization by 8 weeks and motile cilia were observed. Cartilaginous portions of the trachea were maintained in mice receiving fresh transplants, but repopulation of the cartilage was not seen in mice receiving decellularized transplants. We observed superior postsurgical survival, weight gain, and ciliary function in mice receiving fresh transplants compared with those receiving decellularized transplants. The murine orthotopic tracheal transplant provides an appropriate model to assess the repopulation and functional regeneration of decellularized tracheal grafts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.