ArticleThe Journal of general virology2014
Structural constraints in the packaging of bluetongue virus genomic segments.
Article in The Journal of general virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- A Molecular and Structural Perspective on Bluetongue Virus Entry and Assembly.Pathogens (Basel, Switzerland) · 2026Review
- Recruitment of multi-segment genomic RNAs by Bluetongue virus requires a preformed RNA network.Nucleic acids research · 2024Article
- Review
- Prevalence and Strains of Colorado Tick Fever Virus in Rocky Mountain Wood Ticks in the Bitterroot Valley, Montana.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2019Article
- Dynamic network approach for the modelling of genomic sub-complexes in multi-segmented viruses.Nucleic acids research · 2018Article
- Rotavirus Genomic RNA Complex Forms via Specific RNA-RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity.Viruses · 2017Article
- Generation of infectious RNA complexes in Orbiviruses: RNA-RNA interactions of genomic segments.Oncotarget · 2016Article
- Computational and molecular analysis of conserved influenza A virus RNA secondary structures involved in infectious virion production.RNA biology · 2016Article
- Reassortment in segmented RNA viruses: mechanisms and outcomes.Nature reviews. Microbiology · 2016Review
- Disruption of Specific RNA-RNA Interactions in a Double-Stranded RNA Virus Inhibits Genome Packaging and Virus Infectivity.PLoS pathogens · 2015Article
- Sequential packaging of RNA genomic segments during the assembly of Bluetongue virus.Nucleic acids research · 2014Article
- Bluetongue virus capsid assembly and maturation.Viruses · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The mechanism used by bluetongue virus (BTV) to ensure the sorting and packaging of its 10 genomic segments is still poorly understood. In this study, we investigated the packaging constraints for two BTV genomic segments from two different serotypes. Segment 4 (S4) of BTV serotype 9 was mutated sequentially and packaging of mutant ssRNAs was investigated by two newly developed RNA packaging assay systems, one in vivo and the other in vitro. Modelling of the mutated ssRNA followed by biochemical data analysis suggested that a conformational motif formed by interaction of the 5' and 3' ends of the molecule was necessary and sufficient for packaging. A similar structural signal was also identified in S8 of BTV serotype 1. Furthermore, the same conformational analysis of secondary structures for positive-sense ssRNAs was used to generate a chimeric segment that maintained the putative packaging motif but contained unrelated internal sequences. This chimeric segment was packaged successfully, confirming that the motif identified directs the correct packaging of the segment.
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Registered trials
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