Evidence map›Paper›PMID 24979784›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2014

Single-residue insertion switches the quaternary structure and exciton states of cryptophyte light-harvesting proteins.

Stephen J Harrop, Krystyna E Wilk, Rayomond Dinshaw, Elisabetta Collini, Tihana Mirkovic, Chang Ying Teng, Daniel G Oblinsky, Beverley R Green, Kerstin Hoef-Emden, Roger G Hiller and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 82 citations in OpenAlex.

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  15. Photosynthesis tunes quantum-mechanical mixing of electronic and vibrational states to steer exciton energy transfer.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
  16. Article
  17. A far-red cyanobacteriochrome lineage specific for verdins.Proceedings of the National Academy of Sciences of the United States of America · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 4 countries.

Stephen J HarropSchool of Physics, The University of New South Wales, Sydney, NSW 2052, Australia;
Krystyna E WilkSchool of Physics, The University of New South Wales, Sydney, NSW 2052, Australia;
Rayomond DinshawDepartment of Chemistry, University of Toronto, Toronto, ON, Canada M5S 3H6;
Elisabetta ColliniDepartment of Chemical Sciences, University of Padua, 35131 Padua, Italy;
Tihana MirkovicDepartment of Chemistry, University of Toronto, Toronto, ON, Canada M5S 3H6;
Chang Ying TengDepartment of Botany, University of British Columbia, Vancouver, BC, Canada V6T 1Z4;
Daniel G OblinskyDepartment of Chemistry, University of Toronto, Toronto, ON, Canada M5S 3H6;
Beverley R GreenDepartment of Botany, University of British Columbia, Vancouver, BC, Canada V6T 1Z4;
Kerstin Hoef-EmdenBotanical Institute, Cologne Biocenter, University of Cologne, 50674 Cologne, Germany;
Roger G HillerDepartment of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia; and.
Gregory D ScholesDepartment of Chemistry, University of Toronto, Toronto, ON, Canada M5S 3H6;
Paul M G CurmiSchool of Physics, The University of New South Wales, Sydney, NSW 2052, Australia;Centre for Applied Medical Research, St Vincent's Hospital, Sydney, NSW 2010, Australia p.curmi@unsw.edu.au.
University of Toronto · CAUniversity of British Columbia · CAUNSW Sydney · AUMacquarie University · AUSt Vincent's Hospital Sydney · AUUniversity of Cologne · DEUniversity of Padua · IT

Funding

X-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6M
NIGMS NIH HHS P41 GM103393NIGMS NIH HHS P41GM103393
6 · The paper itself

Abstract

Observation of coherent oscillations in the 2D electronic spectra (2D ES) of photosynthetic proteins has led researchers to ask whether nontrivial quantum phenomena are biologically significant. Coherent oscillations have been reported for the soluble light-harvesting phycobiliprotein (PBP) antenna isolated from cryptophyte algae. To probe the link between spectral properties and protein structure, we determined crystal structures of three PBP light-harvesting complexes isolated from different species. Each PBP is a dimer of αβ subunits in which the structure of the αβ monomer is conserved. However, we discovered two dramatically distinct quaternary conformations, one of which is specific to the genus Hemiselmis. Because of steric effects emerging from the insertion of a single amino acid, the two αβ monomers are rotated by ∼73° to an "open" configuration in contrast to the "closed" configuration of other cryptophyte PBPs. This structural change is significant for the light-harvesting function because it disrupts the strong excitonic coupling between two central chromophores in the closed form. The 2D ES show marked cross-peak oscillations assigned to electronic and vibrational coherences in the closed-form PC645. However, such features appear to be reduced, or perhaps absent, in the open structures. Thus cryptophytes have evolved a structural switch controlled by an amino acid insertion to modulate excitonic interactions and therefore the mechanisms used for light harvesting.

Indexed as

Evolution, MolecularModels, MolecularAmino Acid SequenceBase SequenceCryptophytaCrystallography, X-RayDimerizationMolecular Sequence DataMutagenesis, InsertionalPhycobiliproteinsProtein ConformationSequence Analysis, DNASpectrum AnalysisPhycobiliproteinsexcitonic switchingprotein evolutionquantum coherenceX-ray crystallography

Identifiers

PMID24979784
PMCPMC4084447
OpenAlexW2082931421

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.