ArticleProceedings of the National Academy of Sciences of the United States of America2014
SPR-5 and MET-2 function cooperatively to reestablish an epigenetic ground state during passage through the germ line.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 51 citations in OpenAlex.
- Dynamic regulation of H3K9 modifications in cancer: pathogenesis and targeted therapies.Biomarker research · 2026Review
- The DREAM and MEC NuRD complexes reinforce SPR-5/MET-2 maternal reprogramming to maintain the germline-soma distinction.Genetics · 2026Article
- Changing minds epigenetically: Germline genes in neurons disrupt animal behavior.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Ectopic transcription due to inherited histone methylation may interfere with the ongoing function of differentiated neurons.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A unified framework governing the establishment and maintenance of transgenerational epigenetic inheritance.Genetics · 2025Article
- Chromatin and epigenetics in aging biology.Genetics · 2025Review
- Review
- A diet of oxidative stress-adapted bacteria improves stress resistance and lifespan inScience advances · 2024Article
- Sterility in the offspring ofmicroPublication biology · 2024Article
- Inheritance of Stress Responses via Small Non-Coding RNAs in Invertebrates and Mammals.Epigenomes · 2023Review
- Inheritance of associative memories and acquired cellular changes in C. elegans.Nature communications · 2023Article
- SPR-1/CoREST facilitates the maternal epigenetic reprogramming of the histone demethylase SPR-5/LSD1.Genetics · 2023Article
- SETDB1-like MET-2 promotes transcriptional silencing and development independently of its H3K9me-associated catalytic activity.Nature structural & molecular biology · 2022Article
- Gene bookmarking by the heat shock transcription factor programs the insulin-like signaling pathway.Molecular cell · 2021Article
- Article
- Germ granule dysfunction is a hallmark and mirror of Piwi mutant sterility.Nature communications · 2021Article
- Article
- RNAi pathways repress reprogramming of C. elegans germ cells during heat stress.Nucleic acids research · 2020Article
- Hansel, Gretel, and the Consequences of Failing to Remove Histone Methylation Breadcrumbs.Trends in genetics : TIG · 2020Review
- A memory of longevity.eLife · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The Caenorhabditis elegans LSD1 H3K4me2 demethylase SPR-5 reprograms epigenetic transcriptional memory during passage through the germ line. Here we show that mutants in the H3K9me2 methyltransferase, met-2, result in transgenerational epigenetic effects that parallel spr-5 mutants. In addition, we find that spr-5;met-2 double mutants have a synergistic effect on sterility, H3K4me2, and spermatogenesis expression. These results implicate MET-2 as a second histone-modifying enzyme in germ-line reprogramming and suggest a model in which SPR-5 and MET-2 function cooperatively to reestablish an epigenetic ground state required for the continued immortality of the C. elegans germ line. Without SPR-5 and MET-2, we find that the ability to express spermatogenesis genes is transgenerationally passed on to the somatic cells of the subsequent generation. This indicates that H3K4me2 may act in the maintenance of cell fate. Finally, we demonstrate that reducing H3K4me2 causes a large increase in H3K9me2 added by the SPR-5;MET-2 reprogramming mechanism. This finding suggests a novel histone code interaction in which the input chromatin environment dictates the output chromatin state. Taken together, our results provide evidence for a broader reprogramming mechanism in which multiple enzymes coordinately regulate histone information during passage through the germ line.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.