ArticleBiochimica et biophysica acta2014
Calcium-mediated pore expansion and cell death following nanoelectroporation.
Article in Biochimica et biophysica acta, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Coronary Spasm Due to Pulsed Field Ablation: A State-of-the-Art Review.Pacing and clinical electrophysiology : PACE · 2026Review
- Nanosecond pulsed electric fields induce cell-size-dependent selective permeabilization of urothelial cancer cells.Communications biology · 2025Article
- Epithelial-Mesenchymal Transition Increases the Susceptibility of Human A549 Cells to Nanosecond Pulsed Electric Fields.International journal of molecular sciences · 2025Article
- Impact of pulse duration on cardiac electroporation: nanosecond pulses enhance cardiomyocyte selectivity and promote a Raman-detected shift towards apoptotic cell death.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2025Article
- Pulsed field ablation in medicine: irreversible electroporation and electropermeabilization theory and applications.Radiology and oncology · 2025Review
- Power-Driven Electroporation Is Predictive of Treatment Outcomes in a Conductivity-Independent Manner.BME frontiers · 2025Article
- Application of nisin as a potential drug candidate for electrochemotherapy.Frontiers in oncology · 2025Article
- Modulating CaBiophysical journal · 2024Article
- Interplay between Electric Field Strength and Number of Short-Duration Pulses for Efficient Gene Electrotransfer.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Characterization of two distinct immortalized endothelial cell lines, EA.hy926 and HMEC-1, for in vitro studies: exploring the impact of calcium electroporation, CaCell communication and signaling : CCS · 2024Article
- Calcium Electroporation versus Electrochemotherapy with Bleomycin in an In Vivo CAM-Based Uveal Melanoma Xenograft Model.International journal of molecular sciences · 2024Article
- Dynamics of cell membrane lesions and adaptive conductance under the electrical stress.Cell stress · 2024Article
- Effects of Nanosecond Pulsed Electric Field (nsPEF) on a Multicellular Spheroid Tumor Model: Influence of Pulse Duration, Pulse Repetition Rate, Absorbed Energy, and Temperature.International journal of molecular sciences · 2023Article
- Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP).International journal of molecular sciences · 2023Article
- Broadband Electrical Spectroscopy to Distinguish Single-Cell CaSensors (Basel, Switzerland) · 2023Article
- Pulsed Electric Field Ablation of Esophageal Malignancies and Mitigating Damage to Smooth Muscle: An In Vitro Study.International journal of molecular sciences · 2023Article
- Action spectra and mechanisms of (in) efficiency of bipolar electric pulses at electroporation.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- The effectiveness of calcium electroporation combined with gene electrotransfer of a plasmid encoding IL-12 is tumor type-dependent.Frontiers in immunology · 2023Article
- Nanosecond pulsed electric field (nsPEF) and vaccines: a novel technique for the inactivation of SARS-CoV-2 and other viruses?Annals of medicine · 2022Article
- Nanosecond PEF Induces Oxidative Stress and Apoptosis via Proteasomal Activity Inhibition in Gastric Adenocarcinoma Cells with Drug Resistance.International journal of molecular sciences · 2022Article
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Authors and funding
4 authors.
Funding
Abstract
Opening of long-lived pores in the cell membrane is the principal primary effect of intense, nanosecond pulsed electric field (nsPEF). Here we demonstrate that the evolution of pores, cell survival, the time and the mode of cell death (necrotic or apoptotic) are determined by the level of external Ca(2+) after nsPEF. We also introduce a novel, minimally disruptive technique for nsEP exposure of adherent cells on indium tin oxide (ITO)-coated glass coverslips, which does not require cell detachment and enables fast exchanges of bath media. Increasing the Ca(2+) level from the nominal 2-5μM to 2mM for the first 60-90min after permeabilization by 300-nsPEF increased the early (necrotic) death in U937, CHO, and BPAE cells. With nominal Ca(2+), the inhibition of osmotic swelling rescued cells from the early necrosis and increased caspase 3/7 activation later on. However, the inhibition of swelling had a modest or no protective effect with 2mM Ca(2+) in the medium. With the nominal Ca(2+), most cells displayed gradual increase in YO-PRO-1 and propidium (Pr) uptake. With 2mM Ca(2+), the initially lower Pr uptake was eventually replaced by a massive and abrupt Pr entry (necrotic death). It was accompanied by a transient acceleration of the growth of membrane blebs due to the increase of the intracellular osmotic pressure. We conclude that the high-Ca(2+)-dependent necrotic death in nsPEF-treated cells is effected by a delayed, sudden, and osmotically-independent pore expansion (or de novo formation of larger pores), but not by the membrane rupture.
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