ArticleThe Biochemical journal1989
Primary sequence and domain structure of chicken vinculin.
Article in The Biochemical journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 71 citations in OpenAlex.
- Review
- Spatial distribution and functional significance of activated vinculin in living cells.The Journal of cell biology · 2005Article
- Further characterization of the interaction between the cytoskeletal proteins talin and vinculin.The Biochemical journal · 2002Article
- Crystal structure of the M-fragment of alpha-catenin: implications for modulation of cell adhesion.The EMBO journal · 2001Article
- LPP, an actin cytoskeleton protein related to zyxin, harbors a nuclear export signal and transcriptional activation capacity.Molecular biology of the cell · 2000Article
- Vinexin: a novel vinculin-binding protein with multiple SH3 domains enhances actin cytoskeletal organization.The Journal of cell biology · 1999Article
- Vinculin proteolysis unmasks an ActA homolog for actin-based Shigella motility.The Journal of cell biology · 1997Article
- A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family.The EMBO journal · 1997Article
- The focal-adhesion vasodilator-stimulated phosphoprotein (VASP) binds to the proline-rich domain in vinculin.The Biochemical journal · 1996Article
- Acidic phospholipids inhibit the intramolecular association between the N- and C-terminal regions of vinculin, exposing actin-binding and protein kinase C phosphorylation sites.The Biochemical journal · 1996Article
- Vinculin, talin and focal adhesions.Journal of muscle research and cell motility · 1996Review
- Interaction of the 47-kDa talin fragment and the 32-kDa vinculin fragment with acidic phospholipids: a computer analysis.Biophysical journal · 1995Article
- Characterization of an F-actin-binding domain in the cytoskeletal protein vinculin.The Journal of cell biology · 1994Article
- Identification of the vinculin-binding site in the cytoskeletal protein alpha-actinin.The Biochemical journal · 1994Article
- Submembranous junctional plaque proteins include potential tumor suppressor molecules.The Journal of cell biology · 1993Review
- Suppression of vinculin expression by antisense transfection confers changes in cell morphology, motility, and anchorage-dependent growth of 3T3 cells.The Journal of cell biology · 1993Article
- The cytoskeletal protein talin contains at least two distinct vinculin binding domains.The Journal of cell biology · 1993Article
- Expression of chicken vinculin complements the adhesion-defective phenotype of a mutant mouse F9 embryonal carcinoma cell.The Journal of cell biology · 1993Article
- Zyxin and cCRP: two interactive LIM domain proteins associated with the cytoskeleton.The Journal of cell biology · 1992Article
- Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA.The Journal of cell biology · 1992Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have determined the complete sequence of chick vinculin from two overlapping cDNA clones. The vinculin mRNA consists of 262 bp of 5' untranslated sequence, an open reading frame of 3195 bp (excluding the initiation codon) and a long 3' untranslated sequence (greater than 2 kb). Chick vinculin contains 1066 amino acid residues, and has a deduced molecular mass of 116,933 Da. Analysis of the domain structure of vinculin shows that the molecule can be cleaved by V8 proteinase into a 90 kDa globular head and a 32 kDa tail region, the latter of which could further be cleaved into a 27 kDa polypeptide. The 90 kDa globular head contains the N-terminus of vinculin, three 112-residue repeats (residues 259-589), and extends to approximately residue 850. Gel overlay experiments show that it also contains a binding site for the cytoskeletal protein talin. The talin-binding domain was further localized to the N-terminal 398 amino acid residues of the protein by expression in vitro of this region from a vinculin cDNA cloned into the Bluescript SK+ vector. The head and tail domains are apparently separated by a proline-rich region that contains V8-proteinase-cleavage sites and a candidate tyrosine (822)-phosphorylation site. Secondary-structure prediction suggests that the head and tail domains contain alpha-helical regions separated by short stretches of turn/coil. Comparison of the chick with a partial human sequence reveals that vinculin is a highly conserved protein. In chickens Southern-blot analysis is consistent with a single vinculin gene, and it is therefore likely that vinculin, and its higher-molecular-mass isoform termed metavinculin, arise through alternative splicing.
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