ArticleTheScientificWorldJournal2014
Reference gene selection for quantitative real-time RT-PCR normalization in Iris. lactea var. chinensis roots under cadmium, lead, and salt stress conditions.
Article in TheScientificWorldJournal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Screening and Stability Validation of RT-qPCR Reference Genes inInternational journal of molecular sciences · 2026Article
- Reference genes selection and validation in yam by real-time quantitative polymerase chain reaction.Scientific reports · 2025Article
- Article
- Selection and Validation of qRT-PCR Internal Reference Genes to Study Flower Color Formation inInternational journal of molecular sciences · 2024Article
- Article
- Article
- Selection and validation of appropriate reference genes for RT-qPCR analysis of flowering stages and different genotypes of Iris germanica L.Scientific reports · 2021Article
- Candidate reference genes for quantitative gene expression analysis in Lagerstroemia indica.Molecular biology reports · 2021Article
- Systems biology reveals key tissue-specific metabolic and transcriptional signatures involved in the response ofComputational and structural biotechnology journal · 2021Article
- Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf.Molecular biology reports · 2020Article
- Early Transcriptional Response to DNA Virus Infection inViruses · 2019Article
- Transcriptome Profiling of Louisiana iris Root and Identification of Genes Involved in Lead-Stress Response.International journal of molecular sciences · 2015Article
- Identification of a novel reference gene for apple transcriptional profiling under postharvest conditions.PloS one · 2015Article
- Role of Ulva lactuca extract in alleviation of salinity stress on wheat seedlings.TheScientificWorldJournal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative real time PCR (RT-qPCR) has emerged as an accurate and sensitive method to measure the gene expression. However, obtaining reliable result depends on the selection of reference genes which normalize differences among samples. In this study, we assessed the expression stability of seven reference genes, namely, ubiquitin-protein ligase UBC9 (UBC), tubulin alpha-5 (TUBLIN), eukaryotic translation initiation factor (EIF-5A), translation elongation factor EF1A (EF1 α ), translation elongation factor EF1B (EF1b), actin11 (ACTIN), and histone H3 (HIS), in Iris. lactea var. chinensis (I. lactea var. chinensis) root when the plants were subjected to cadmium (Cd), lead (Pb), and salt stress conditions. All seven reference genes showed a relatively wide range of threshold cycles (C t ) values in different samples. GeNorm and NormFinder algorithms were used to assess the suitable reference genes. The results from the two software units showed that EIF-5A and UBC were the most stable reference genes across all of the tested samples, while TUBLIN was unsuitable as internal controls. I. lactea var. chinensis is tolerant to Cd, Pb, and salt. Our results will benefit future research on gene expression in response to the three abiotic stresses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.