ArticleBiotechnology for biofuels2014
Suitability of magnetic nanoparticle immobilised cellulases in enhancing enzymatic saccharification of pretreated hemp biomass.
Article in Biotechnology for biofuels, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 264 citations in OpenAlex.
- Layered Polymeric Carbon Nitride as a Green Support for Cellulase Immobilization: Improved Stability, Activity, and Reusability.Applied biochemistry and biotechnology · 2026Article
- A Multienzyme Magnetic Nanocatalyst for Efficient, Sustainable Hydrolysis of Wastewater-Grown Microalgae Consortia.ACS sustainable chemistry & engineering · 2026Article
- Green synthesized Fe nanoparticle assisted biomass hydrolysis for bioenergy production: process parameters optimization through combined RSM and ANN based approach.Journal of environmental health science & engineering · 2025Article
- Green synthesis of magnetic bio-Graphene nanohybrid for the immobilization of hydrolytic enzymes towards sustainable bioconversion of cellulose.RSC advances · 2025Article
- Biofunctionalization of Magneto-Plasmonic FeMolecules (Basel, Switzerland) · 2025Article
- Production of Alginate Oligosaccharides (AOSs) Using Enhanced Physicochemical Properties of Immobilized Alginate Lyase for Industrial Application.Marine drugs · 2024Article
- Immobilization and Characterization of a Processive Endoglucanase EG5C-1 fromNanomaterials (Basel, Switzerland) · 2024Article
- Recent advances and challenges in the utilization of nanomaterials in transesterification for biodiesel production.Heliyon · 2023Review
- (Magnetic) Cross-Linked Enzyme Aggregates of Cellulase fromMolecules (Basel, Switzerland) · 2023Article
- Development of sustainable downstream processing for nutritional oil production.Frontiers in bioengineering and biotechnology · 2023Review
- Lignocellulose dissociation with biological pretreatment towards the biochemical platform: A review.Materials today. Bio · 2022Review
- Perspectives on Nanomaterials and Nanotechnology for Sustainable Bioenergy Generation.Materials (Basel, Switzerland) · 2022Review
- Biotechnological advances in biomass pretreatment for bio-renewable production through nanotechnological intervention.Biomass conversion and biorefinery · 2022Review
- Improvement of Ruminal Neutral Detergent Fiber Degradability by Obtaining and Using Exogenous Fibrolytic Enzymes from White-Rot Fungi.Animals : an open access journal from MDPI · 2022Review
- Industrial applications of immobilized nano-biocatalysts.Bioprocess and biosystems engineering · 2022Review
- Current perspective on production and applications of microbial cellulases: a review.Bioresources and bioprocessing · 2021Review
- Immobilization of endoglucanase on kaolin by adsorption and covalent bonding.Bioprocess and biosystems engineering · 2021Article
- Immobilization of Laccase on Magnetic Nanoparticles and Application in the Detoxification of Rice Straw Hydrolysate for the Lipid Production of Rhodotorula glutinis.Applied biochemistry and biotechnology · 2021Article
- A novel all-in-one strategy for purification and immobilization of β-1,3-xylanase directly from cell lysate as active and recyclable nanobiocatalyst.Microbial cell factories · 2021Article
- Enzyme-functionalised, core/shell magnetic nanoparticles for selective pH-triggered sucrose capture.RSC advances · 2021Article
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4 authors at 1 institution in 1 country.
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Abstract
backgroundPrevious research focused on pretreatment of biomass, production of fermentable sugars and their consumption to produce ethanol. The main goal of the work was to economise the production process cost of fermentable sugars. Therefore, the objective of the present work was to investigate enzyme hydrolysis of microcrystalline cellulose and hemp hurds (natural cellulosic substrate) using free and immobilised enzymes. Cellulase from Trichoderma reesei was immobilised on an activated magnetic support by covalent binding and its activity was compared with that of the free enzyme to hydrolyse microcrystalline cellulose and hemp hurds on the basis of thermostability and reusability.
resultsUp to 94% protein binding was achieved during immobilisation of cellulase on nanoparticles. Successful binding was confirmed using Fourier transform infrared spectroscopy (FTIR). The free and immobilised enzymes exhibited identical pH optima (pH 4.0) and differing temperature optima at 50°C and 60°C, respectively. The K M values obtained for the free and immobilised enzymes were 0.87 mg/mL and 2.6 mg/mL respectively. The immobilised enzyme retained 50% enzyme activity up to five cycles, with thermostability at 80°C superior to that of the free enzyme. Optimum hydrolysis of carboxymethyl cellulose (CMC) with free and immobilised enzymes was 88% and 81%, respectively. With pretreated hemp hurd biomass (HHB), the free and immobilised enzymes resulted in maximum hydrolysis in 48 h of 89% and 93%, respectively.
conclusionThe current work demonstrated the advantages delivered by immobilised enzymes by minimising the consumption of cellulase during substrate hydrolysis and making the production process of fermentable sugars economical and feasible. The activity of cellulase improved as a result of the immobilisation, which provided a better stability at higher temperatures. The immobilised enzyme provided an advantage over the free enzyme through the reusability and longer storage stability properties that were gained as a result of the immobilisation.
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