ArticleJournal of molecular signaling2014
Breast cancer cell invasion mediated by Gα12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2.
Article in Journal of molecular signaling, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
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- Association between matrix metalloproteinase-9 gene polymorphism and breast cancer in Brazilian women.Clinics (Sao Paulo, Brazil) · 2020Article
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- GNA13 promotes tumor growth and angiogenesis by upregulating CXC chemokines via the NF-κB signaling pathway in colorectal cancer cells.Cancer medicine · 2018Article
- Study on the expression of p53 and MMP-2 in patients with lung cancer after interventional therapy.Oncology letters · 2018Article
- Silence of α1-Antitrypsin Inhibits Migration and Proliferation of Triple Negative Breast Cancer Cells.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Effects of various treatment approaches for treatment efficacy for late stage breast cancer and expression level of TIMP-1 and MMP-9.Cancer biomarkers : section A of Disease markers · 2018Article
- Expression of Th17/Treg related molecules in gastric cancer tissues.The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology · 2018Article
- The relationship between MMP-2 and MMP-9 expression levels with breast cancer incidence and prognosis.Oncology letters · 2017Article
- Extracting Intercellular Signaling Network of Cancer Tissues using Ligand-Receptor Expression Patterns from Whole-tumor and Single-cell Transcriptomes.Scientific reports · 2017Article
- Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situ.Breast cancer research : BCR · 2017Article
- c-Jun Contributes to Transcriptional Control of GNA12 Expression in Prostate Cancer Cells.Molecules (Basel, Switzerland) · 2017Article
- Data-Driven Discovery of Extravasation Pathway in Circulating Tumor Cells.Scientific reports · 2017Article
- Article
- miR-564 acts as a dual inhibitor of PI3K and MAPK signaling networks and inhibits proliferation and invasion in breast cancer.Scientific reports · 2016Article
- Mast Cell Targeted Chimeric Toxin Can Be Developed as an Adjunctive Therapy in Colon Cancer Treatment.Toxins · 2016Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecent studies on the involvement of the G12 family of heterotrimeric G proteins (Gα12 and Gα13, the products of the GNA12 and GNA13 genes, respectively) in oncogenic pathways have uncovered a link between G12 signaling and cancer progression. However, despite a well characterized role of Rho GTPases, the potential role of secreted factors in the capacity of G12 signaling to promote invasion of cancer cells is just beginning to be addressed.
methodsMDA-MB-231 and MCF10A breast cancer cell lines were employed as a model system to explore the involvement of secreted factors in G12-stimulated cell invasion. Factors secreted by cells expressing dominant-active Gα12 were identified by protein array, and their involvement in breast cancer cell invasion was assessed through both RNAi-mediated knockdown and antibody neutralization approaches. Bioinformatics analysis of the promoter elements of the identified factors suggested NF-κB elements played a role in their enhanced expression, which was tested by chromatin immunoprecipitation.
resultsWe found that signaling through the Gα12 in MDA-MB-231 and MCF10A breast cancer cell lines enhances expression of interleukins (IL)-6 and -8, and matrix metalloproteinase (MMP)-2, and that these secreted factors play a role in G12-stimulated cell invasion. Furthermore, the enhanced expression of these secreted factors was found to be facilitated by the activation of their corresponding promoters, where NF-κB seems to be one of the major regulators. Inhibition of IL-6 and IL-8, or MMP-2 activity significantly decreased Gα12-mediated cell invasion.
conclusionsThese studies confirm and extend findings that secreted factors contribute to the oncogenic potential of G12 signaling, and suggest potential therapeutic targets to control this process.
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