ArticleMediators of inflammation2014
Biomechanical loading modulates proinflammatory and bone resorptive mediators in bacterial-stimulated PDL cells.
Article in Mediators of inflammation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Mechanical force application and inflammation induce osteoclastogenesis by independent pathways.Clinical oral investigations · 2023Article
- Effect of Different Parameters of In Vitro Static Tensile Strain on Human Periodontal Ligament Cells Simulating the Tension Side of Orthodontic Tooth Movement.International journal of molecular sciences · 2022Article
- Cyclic tensile strain affects the response of human periodontal ligament stromal cells to tumor necrosis factor-α.Clinical oral investigations · 2022Article
- Biomechanical Properties of Bone and Mucosa for Design and Application of Dental Implants.Materials (Basel, Switzerland) · 2021Article
- Effect of Tension on Human Periodontal Ligament Cells: Systematic Review and Network Analysis.Frontiers in bioengineering and biotechnology · 2021Article
- Current Trends in In Vitro Modeling to Mimic Cellular Crosstalk in Periodontal Tissue.Advanced healthcare materials · 2021Review
- Regulation of the autophagy-marker Sequestosome 1 in periodontal cells and tissues by biomechanical loading.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2020Article
- The role of mechanotransduction versus hypoxia during simulated orthodontic compressive strain-an in vitro study of human periodontal ligament fibroblasts.International journal of oral science · 2019Article
- Persistent Exposure toFrontiers in cellular and infection microbiology · 2019Article
- Diets That Promote Colon Inflammation Associate With Risk of Colorectal Carcinomas That Contain Fusobacterium nucleatum.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2018Article
- Mechanical loading increases pro-inflammatory effects of nitrogen-containing bisphosphonate in human periodontal fibroblasts.Clinical oral investigations · 2018Article
- Understanding biomaterial-tissue interface quality: combinedScience and technology of advanced materials · 2017Review
- Regulation of Ghrelin Receptor by Periodontal BacteriaMediators of inflammation · 2017Article
- Low magnitude high frequency vibration induces RANKL via cyclooxygenase pathway in human periodontal ligament cellsJournal of oral biology and craniofacial researchArticle
- RANK-RANKL-OPG expression after gingival mesenchymal stem cell hypoxia preconditioned application in an orthodontic tooth movement animal model.Journal of oral biology and craniofacial researchArticle
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Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (PDL) cells in the presence of bacterial challenge. Cells were seeded on BioFlex culture plates and exposed to Fusobacterium nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL) and high (CTSH) magnitudes for 1 and 3 days. Synthesis of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) was evaluated by ELISA. Gene expression and protein secretion of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) were evaluated by quantitative RT-PCR and ELISA, respectively. F. nucleatum increased the production of COX2 and PGE2, which was further increased by CTS. F. nucleatum-induced increase of PGE2 synthesis was significantly (P < 0.05) increased when CTSH was applied at 1 and 3 days. In addition, CTSH inhibited the F. nucleatum-induced upregulation of OPG at 1 and 3 days, thereby increasing the RANKL/OPG ratio. OPG and RANKL mRNA results correlated with the protein results. In summary, our findings provide original evidence that CTS can enhance bacterial-induced syntheses of molecules associated with inflammation and bone resorption by PDL cells. Therefore, biomechanical, such as orthodontic or occlusal, loading may enhance the bacterial-induced inflammation and destruction in periodontitis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.