Evidence map›Paper›PMID 24976626›Full record

ArticleClinical biochemistry2014

Exosomal transfer from human renal proximal tubule cells to distal tubule and collecting duct cells.

John J Gildea, Joscelyn E Seaton, Ken G Victor, Camellia M Reyes, Dora Bigler Wang, Abigail C Pettigrew, Crystal E Courtner, Neema Shah, Hanh T Tran, Robert E Van Sciver and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed, 1 pooled it
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed, 1 synthesis or guideline pooled it, 113 citations in OpenAlex.

  1. Guideline
  2. Review
  3. Article
  4. Review
  5. Review
  6. The role of extracellular vesicles in kidney disease progression.Kidney research and clinical practice · 2026
    Article
  7. Review
  8. Article
  9. Review
  10. Cell-cell communication in kidney fibrosis.Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association · 2024
    Review
  11. Review
  12. Review
  13. Review
  14. Acute Kidney Injury by Ischemia/Reperfusion and Extracellular Vesicles.International journal of molecular sciences · 2023
    Review
  15. Article
  16. Extracellular Vesicle MicroRNA in the Kidney.Comprehensive Physiology · 2023
    Article
  17. Extracellular vesicles as personalized medicine.Molecular aspects of medicine · 2023
    Review
  18. Article
  19. Article
  20. Review

14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

John J GildeaThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA. Electronic address: jjg5b@virginia.edu.
Joscelyn E SeatonThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Ken G VictorThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Camellia M ReyesThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Dora Bigler WangThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Abigail C PettigrewThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Crystal E CourtnerThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Neema ShahThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Hanh T TranThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Robert E Van SciverThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Julia M CarlsonThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
Robin A FelderThe University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA.
University of Virginia Health System · US

Funding

Spatiotemporal transregulation of D1-like angiotensin receptors in genetically...P01HL074940 · NHLBI · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI GILDEA, JOHN JOSEPH · 2004 to 2020
$31.8M
Renal Vascular Oxidative Stress in HypertensionP01HL068686 · NHLBI · GEORGETOWN UNIVERSITY · PI JOSE, PEDRO A., SANDBERG, KATHRYN L · 2001 to 2017
$28.8M
RENAL DOPAMINE-1 RECEPTOR DEFECT IN HYPERTENSIONR01DK039308 · NIDDK · UNIVERSITY OF MARYLAND BALTIMORE · PI JOSE, PEDRO A. · 1987 to 2020
$7.4M
NHLBI NIH HHS HL074940NHLBI NIH HHS P01 HL068686NHLBI NIH HHS P01 HL074940NIDDK NIH HHS DK039308NIDDK NIH HHS R01 DK039308
6 · The paper itself

Abstract

objectivesExosomes are 50-90nm extracellular membrane particles that may mediate trans-cellular communication between cells and tissues. We have reported that human urinary exosomes contain miRNA that are biomarkers for salt sensitivity and inverse salt sensitivity of blood pressure. This study examines exosomal transfer between cultured human renal proximal tubule cells (RPTCs) and from RPTCs to human distal tubule and collecting duct cells. DESIGN AND

methodsFor RPTC-to-RPTC exosomal transfer, we utilized 5 RPTC lines producing exosomes that were fluorescently labeled with exosomal-specific markers CD63-EGFP or CD9-RFP. Transfer between RPTCs was demonstrated by co-culturing CD63-EGFP and CD9-RFP stable clones and performing live confocal microscopy. For RPTC-to-distal segment exosomal transfer, we utilized 5 distal tubule and 3 collecting duct immortalized cell lines.

resultsTime-lapse videos revealed unique proximal tubule cellular uptake patterns for exosomes and eventual accumulation into the multivesicular body. Using culture supernatant containing exosomes from 3 CD9-RFP and 2 CD63-EGFP RPTC cell lines, all 5 distal tubule cell lines and all 3 collecting duct cell lines showed exosomal uptake as measured by microplate fluorometry. Furthermore, we found that RPTCs stimulated with fenoldopam (dopamine receptor agonist) had increased production of exosomes, which upon transfer to distal tubule and collecting duct cells, reduced the basal reactive oxygen species (ROS) production rates in those recipient cells.

conclusionDue to the complex diversity of exosomal contents, this proximal-to-distal vesicular inter-nephron transfer may represent a previously unrecognized trans-renal communication system.

Indexed as

Cell CommunicationCell LineExosomesHumansKidneyKidney Tubules, CollectingKidney Tubules, DistalKidney Tubules, ProximalMicroRNAsNephronsTetraspanin 29Tetraspanin 30CD63 protein, humanCD9 protein, humanMicroRNAsTetraspanin 29Tetraspanin 30Collecting ductDistal tubuleDopamineExosomesProximal tubuleROS

Identifiers

PMID24976626
PMCPMC5384615
OpenAlexW1973102509

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.