ArticleMolecular biology reports2014
Identification and transcript analysis of two glutamate decarboxylase genes, CsGAD1 and CsGAD2, reveal the strong relationship between CsGAD1 and citrate utilization in citrus fruit.
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 65 citations in OpenAlex.
- Decoding the multifunctional role of γ-aminobutyric acid in plant adaptation to drought stress.Planta · 2026Review
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- Transcriptome Analysis and VIGS Identification of Key Genes Regulating Citric Acid Metabolism in Citrus.Current issues in molecular biology · 2023Article
- Acetylome reprograming participates in the establishment of fruit metabolism during polyploidization in citrus.Plant physiology · 2022Article
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- Genome-wide identification of GAD family genes suggestsFrontiers in genetics · 2022Article
- Metabolomic and transcriptomic analyses reveal the mechanism of sweet-acidic taste formation during pineapple fruit development.Frontiers in plant science · 2022Article
- Comprehensive Genome-Wide Identification and Transcript Profiling of GABA Pathway Gene Family in Apple (Genes · 2021Article
- High-spatiotemporal-resolution transcriptomes provide insights into fruit development and ripening in Citrus sinensis.Plant biotechnology journal · 2021Article
- Gamma-aminobutyric acid (GABA) alleviates salt damage in tomato by modulating NaBMC plant biology · 2020Article
- Comparative Metabolites and Citrate-Degrading Enzymes Activities in Citrus Fruits Reveal the Role of Balance between ACL and Cyt-ACO in Metabolite Conversions.Plants (Basel, Switzerland) · 2020Article
- Dissection of the Mechanism for Compatible and Incompatible Graft Combinations of Citrus grandis (L.) Osbeck ('Hongmian Miyou').International journal of molecular sciences · 2018Article
- Identification of twoPeerJ · 2017Article
- Identifying novel fruit-related genes in Arabidopsis thaliana based on the random walk with restart algorithm.PloS one · 2017Article
- Transcriptome Changes Associated with Boron Deficiency in Leaves of Two Citrus Scion-Rootstock Combinations.Frontiers in plant science · 2017Article
- Identification and transcript profiles of citrus growth-regulating factor genes involved in the regulation of leaf and fruit development.Molecular biology reports · 2016Article
- Citrate Accumulation-Related Gene Expression and/or Enzyme Activity Analysis Combined With Metabolomics Provide a Novel Insight for an Orange Mutant.Scientific reports · 2016Article
- The Citrus transcription factor, CitERF13, regulates citric acid accumulation via a protein-protein interaction with the vacuolar proton pump, CitVHA-c4.Scientific reports · 2016Article
- Proteomics and SSH Analyses of ALA-Promoted Fruit Coloration and Evidence for the Involvement of a MADS-Box Gene,Frontiers in plant science · 2016Article
- Integrated Systems Biology Analysis of Transcriptomes Reveals Candidate Genes for Acidity Control in Developing Fruits of Sweet Orange (Citrus sinensis L. Osbeck).Frontiers in plant science · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutamate decarboxylase (GAD, EC 4.1.1.15) has been suggested to be a key, regulatory point in the biosynthesis of γ-aminobutyrate (GABA) and in the utilization of citric acid through GABA shunt pathway. In this study we discovered two GAD genes, named as CsGAD1 and CsGAD2, in citrus genome database and then successfully cloned. Both CsGAD1 and CsGAD2 have a putative pyridoxal 5-phosphate binding domain in the middle region and a putative calmodulin-binding domain at the carboxyl terminus. Gene structure analysis showed that much difference exists in the size of exons and introns or in cis-regulatory elements in promoter region between the two GAD genes. Gene expression indicated that CsGAD1 transcript was predominantly expressed in flower and CsGAD2 transcript was predominantly expressed in fruit juice sacs; in the ripening fruit, CsGAD1 transcript level was at least 2-time higher than CsGAD2 transcript level. Moreover, CsGAD1 transcript level was increased significantly along with the increase of GAD activity and accompanied by a significant decrease of titratable acid (TA), suggesting that it is CsGAD1 rather than CsGAD2 plays a role in the citric acid utilization during fruit ripening. In addition, injection of abscisic acid and foliar spray of K2SO4 significantly increased the TA content of Satsuma mandarin, and significantly decreased GAD activity as well as CsGAD1 transcript, further suggesting the important role of CsGAD1 in the citrate utilization of citrus fruit.
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