ArticleScientific reports2014
Network dynamics of 3D engineered neuronal cultures: a new experimental model for in-vitro electrophysiology.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed.
- Three-dimensional microelectrode arrays forBiomedical engineering letters · 2026Review
- Ex vivo engineering of neural tissue structure and growth using sequential 2D and 3D solid scaffolds.In vitro cellular & developmental biology. Animal · 2026Article
- Monolithic 3D Nanoelectrode Arrays on CMOS Circuitry for Scalable, High-Resolution Neural Recording.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Patient-derived brain organoids reveal divergent neuronal activity across subpopulations of autism spectrum disorder.Translational psychiatry · 2026Article
- Recent Advances in Microelectrode Array Interfaces for Organoids.Biomimetics (Basel, Switzerland) · 2026Review
- GLUT1-DS Brain Organoids Exhibit Increased Sensitivity to Metabolic and Pharmacological Induction of Epileptiform Activity.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Engineering 3D neural models of neurodegeneration: the Neural Microenvironment Design Code (NMDC), design principles, and emerging new approach methodologies.NAM journal · 2026Review
- Mechanodynamic brain on chip for studying human stem cell derived neuronal networks.Scientific reports · 2025Article
- Computational analysis of 3D biopolymeric porous scaffolds for the in vitro development of neural networks.PloS one · 2025Article
- Enhanced electrophysiological recordings in acute brain slices, spheroids, and organoids using 3D high-density multielectrode arrays.PloS one · 2025Article
- Human Pluripotent Stem Cell-Derived Astrocyte Functionality Compares Favorably with Primary Rat Astrocytes.eNeuro · 2024Article
- Electrophysiological features of cortical 3D networks are deeply modulated by scaffold properties.APL bioengineering · 2024Article
- Epileptiform activity in brain organoids derived from patient with Glucose Transporter 1 Deficiency Syndrome.Frontiers in neuroscience · 2024Article
- Versatile micro-electrode array to monitor human iPSC derived 3D neural tissues at air-liquid interface.Frontiers in cellular neuroscience · 2024Article
- PEDOT:PSS-coated platinum electrodes for neural stimulation.APL bioengineering · 2023Article
- Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels.Gels (Basel, Switzerland) · 2023Article
- Portrait of intense communications within microfluidic neural networks.Scientific reports · 2023Article
- Human-Derived Cortical Neurospheroids Coupled to Passive, High-Density and 3D MEAs: A Valid Platform for Functional Tests.Bioengineering (Basel, Switzerland) · 2023Article
- Modeling the three-dimensional connectivity of in vitro cortical ensembles coupled to Micro-Electrode Arrays.PLoS computational biology · 2023Article
- Spatiotemporal analysis of 3D human iPSC-derived neural networks using a 3D multi-electrode array.Frontiers in cellular neuroscience · 2023Article
6 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
Despite the extensive use of in-vitro models for neuroscientific investigations and notwithstanding the growing field of network electrophysiology, all studies on cultured cells devoted to elucidate neurophysiological mechanisms and computational properties, are based on 2D neuronal networks. These networks are usually grown onto specific rigid substrates (also with embedded electrodes) and lack of most of the constituents of the in-vivo like environment: cell morphology, cell-to-cell interaction and neuritic outgrowth in all directions. Cells in a brain region develop in a 3D space and interact with a complex multi-cellular environment and extracellular matrix. Under this perspective, 3D networks coupled to micro-transducer arrays, represent a new and powerful in-vitro model capable of better emulating in-vivo physiology. In this work, we present a new experimental paradigm constituted by 3D hippocampal networks coupled to Micro-Electrode-Arrays (MEAs) and we show how the features of the recorded network dynamics differ from the corresponding 2D network model. Further development of the proposed 3D in-vitro model by adding embedded functionalized scaffolds might open new prospects for manipulating, stimulating and recording the neuronal activity to elucidate neurophysiological mechanisms and to design bio-hybrid microsystems.
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