ArticleMolecular microbiology2014
Bacterial sugar utilization gives rise to distinct single-cell behaviours.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Overexpression of human interleukin 6 in NaCl inducible bacterial system and its characterization.Applied biochemistry and biotechnology · 2026Article
- Single-cell detection and quantification of the microbiota by MicFLY.Cell host & microbe · 2026Article
- Sugar-inducible promoters for manipulation of core metabolic pathways in the thermophilic acetogenApplied and environmental microbiology · 2025Article
- Single-cell quantification of the microbiota by flow cytometry: MicFLY.bioRxiv : the preprint server for biology · 2025Article
- Microbiota transplantation for cotton leaf curl disease suppression-core microbiome and transcriptome dynamics.Communications biology · 2025Article
- Single-cell data reveal heterogeneity of investment in ribosomes across a bacterial population.Nature communications · 2025Article
- A single vector system for tunable and homogeneous dual gene expression in Escherichia coli.Scientific reports · 2025Article
- Irreversibility in bacterial regulatory networks.Science advances · 2024Article
- RNA recording in single bacterial cells using reprogrammed tracrRNAs.Nature biotechnology · 2023Article
- Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9.Science (New York, N.Y.) · 2021Article
- Sequence-independent RNA sensing and DNA targeting by a split domain CRISPR-Cas12a gRNA switch.Nucleic acids research · 2021Article
- Tunable expression rate control of a growth-decoupled T7 expression system by L-arabinose only.Microbial cell factories · 2021Article
- Subpopulation behaviors in lactose metabolism by Streptococcus mutans.Molecular microbiology · 2021Article
- Impact of the Expression System on Recombinant Protein Production inFrontiers in microbiology · 2021Article
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- Improvement ofMicroorganisms · 2020Article
- Escherichia coli σMicrobial cell factories · 2020Article
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- Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis.Nature communications · 2019Article
- The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays.RNA biology · 2019Article
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4 authors.
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Abstract
Inducible utilization pathways reflect widespread microbial strategies to uptake and consume sugars from the environment. Despite their broad importance and extensive characterization, little is known how these pathways naturally respond to their inducing sugar in individual cells. Here, we performed single-cell analyses to probe the behaviour of representative pathways in the model bacterium Escherichia coli. We observed diverse single-cell behaviours, including uniform responses (d-lactose, d-galactose, N-acetylglucosamine, N-acetylneuraminic acid), 'all-or-none' responses (d-xylose, l-rhamnose) and complex combinations thereof (l-arabinose, d-gluconate). Mathematical modelling and probing of genetically modified pathways revealed that the simple framework underlying these pathways - inducible transport and inducible catabolism - could give rise to most of these behaviours. Sugar catabolism was also an important feature, as disruption of catabolism eliminated tunable induction as well as enhanced memory of previous conditions. For instance, disruption of catabolism in pathways that respond to endogenously synthesized sugars led to full pathway induction even in the absence of exogenous sugar. Our findings demonstrate the remarkable flexibility of this simple biological framework, with direct implications for environmental adaptation and the engineering of synthetic utilization pathways as titratable expression systems and for metabolic engineering.
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