ArticleJournal of molecular biology2014
The presence of an RNA:DNA hybrid that is prone to slippage promotes termination by T7 RNA polymerase.
Article in Journal of molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Engineering the poly(A) tail for therapeutic mRNA: from expression control to manufacturing robustness.Frontiers in bioengineering and biotechnology · 2026Review
- Nascent RNA profiling reveals genome-wide productive reiterative initiation regulating gene transcription in bacteria.Nucleic acids research · 2025Article
- Characterisation and analysis of mRNA critical quality attributes using liquid chromatography based methods.Journal of chromatography. A · 2025Review
- Single-mode termination of phage transcriptions, disclosing bacterial adaptation for facilitated reinitiations.Nucleic acids research · 2024Article
- Novel Mobile Phase to Control Charge States and Metal Adducts in the LC/MS for mRNA Characterization Assays.ACS omega · 2022Article
- Mechanisms of mitochondrial promoter recognition in humans and other mammalian species.Nucleic acids research · 2022Article
- Massively parallel characterization of engineered transcript isoforms using direct RNA sequencing.Nature communications · 2022Article
- Structural basis of reiterative transcription from the pyrG and pyrBI promoters by bacterial RNA polymerase.Nucleic acids research · 2020Article
- Propensity of a picornavirus polymerase to slip on potyvirus-derived transcriptional slippage sites.The Journal of general virology · 2019Article
- Non-programmed transcriptional frameshifting is common and highly RNA polymerase type-dependent.Microbial cell factories · 2018Article
- X-ray crystal structure of a reiterative transcription complex reveals an atypical RNA extension pathway.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Mutational analysis of the Potyviridae transcriptional slippage site utilized for expression of the P3N-PIPO and P1N-PISPO proteins.Nucleic acids research · 2016Article
- The P1N-PISPO trans-Frame Gene of Sweet Potato Feathery Mottle Potyvirus Is Produced during Virus Infection and Functions as an RNA Silencing Suppressor.Journal of virology · 2016Article
- Transcriptional slippage in the positive-sense RNA virus family Potyviridae.EMBO reports · 2015Article
- Mechanism of Transcription Termination by RNA Polymerase III Utilizes a Non-template Strand Sequence-Specific Signal Element.Molecular cell · 2015Article
- Productive mRNA stem loop-mediated transcriptional slippage: Crucial features in common with intrinsic terminators.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Intrinsic termination signals for multisubunit bacterial RNA polymerases (RNAPs) encode a GC-rich stem-loop structure followed by a polyuridine [poly(U)] tract, and it has been proposed that steric clash of the stem-loop with the exit pore of the RNAP imposes a shearing force on the RNA in the downstream RNA:DNA hybrid, resulting in misalignment of the active site. The structurally unrelated T7 RNAP terminates at a similar type of signal (TΦ), suggesting a common mechanism for termination. In the absence of a hairpin (passive conditions), T7 RNAP slips efficiently in both homopolymeric A and U tracts, and we have found that replacement of the U tract in TΦ with a slippage-prone A tract still allows efficient termination. Under passive conditions, incorporation of a single G residue following a poly(U) tract (which is the situation during termination at TΦ) results in a "locked" complex that is unable to extend the transcript. Our results support a model in which transmission of the shearing force generated by steric clash of the hairpin with the exit pore is promoted by the presence of a slippery tracts downstream, resulting in alterations in the active site and the formation of a locked complex that represents an early step in the termination pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.