ArticleAmerican journal of human genetics2014
Integrative DNA, RNA, and protein evidence connects TREML4 to coronary artery calcification.
Article in American journal of human genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Host-Filtered Blood Nucleic Acids for Pathogen Detection: Shared Background, Sparse Signal, and Methodological Limits.Pathogens (Basel, Switzerland) · 2026Article
- Decoding coronary artery calcification: metabolic reprogramming features and a promising circulating biomarker PXDN.Frontiers in cell and developmental biology · 2026Article
- The biology of TREM receptors.Nature reviews. Immunology · 2023Review
- TREML4 polymorphisms increase the mRNA in blood leukocytes in the progression of atherosclerosis.Scientific reports · 2022Article
- Signature transcriptome analysis of stage specific atherosclerotic plaques of patients.BMC medical genomics · 2022Article
- Presence of periodontal pathogenic bacteria in blood of patients with coronary artery disease.Scientific reports · 2022Article
- Macrophages in Atherosclerosis, First or Second Row Players?Biomedicines · 2021Review
- Overlapping Genetic Background of Coronary Artery and Carotid/Femoral Atherosclerotic Calcification.Medicina (Kaunas, Lithuania) · 2021Article
- Article
- Weighed Gene Coexpression Network Analysis Screens the Potential Long Noncoding RNAs and Genes Associated with Progression of Coronary Artery Disease.Computational and mathematical methods in medicine · 2020Article
- Article
- TREML4 mRNA Expression and Polymorphisms in Blood Leukocytes are Associated with Atherosclerotic Lesion Extension in Coronary Artery Disease.Scientific reports · 2019Article
- Cardiovascular calcification: artificial intelligence and big data accelerate mechanistic discovery.Nature reviews. Cardiology · 2019Review
- Genetics of Subclinical Coronary Atherosclerosis.Current genetic medicine reports · 2018Article
- Genotype-driven identification of a molecular network predictive of advanced coronary calcium in ClinSeq® and Framingham Heart Study cohorts.BMC systems biology · 2017Article
- Autocrine IL-10 functions as a rheostat for M1 macrophage glycolytic commitment by tuning nitric oxide production.Redox biology · 2016Article
- Genetics and Genomics of Coronary Artery Disease.Current cardiology reports · 2016Review
- Systematic Evaluation of Sanger Validation of Next-Generation Sequencing Variants.Clinical chemistry · 2016Article
- Genomic Modifiers of Natural Killer Cells, Immune Responsiveness and Lymphoid Tissue Remodeling Together Increase Host Resistance to Viral Infection.PLoS pathogens · 2016Article
- The receptor TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity.Nature immunology · 2015Article
Corrections and comments
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Authors and funding
32 authors at 10 institutions in 2 countries.
Funding
Abstract
Coronary artery calcification (CAC) is a heritable and definitive morphologic marker of atherosclerosis that strongly predicts risk for future cardiovascular events. To search for genes involved in CAC, we used an integrative transcriptomic, genomic, and protein expression strategy by using next-generation DNA sequencing in the discovery phase with follow-up studies using traditional molecular biology and histopathology techniques. RNA sequencing of peripheral blood from a discovery set of CAC cases and controls was used to identify dysregulated genes, which were validated by ClinSeq and Framingham Heart Study data. Only a single gene, TREML4, was upregulated in CAC cases in both studies. Further examination showed that rs2803496 was a TREML4 cis-eQTL and that the minor allele at this locus conferred up to a 6.5-fold increased relative risk of CAC. We characterized human TREML4 and demonstrated by immunohistochemical techniques that it is localized in macrophages surrounding the necrotic core of coronary plaques complicated by calcification (but not in arteries with less advanced disease). Finally, we determined by von Kossa staining that TREML4 colocalizes with areas of microcalcification within coronary plaques. Overall, we present integrative RNA, DNA, and protein evidence implicating TREML4 in coronary artery calcification. Our findings connect multimodal genomics data with a commonly used clinical marker of cardiovascular disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.