ArticleMolecular biology reports2014
Identification of suitable endogenous control genes for quantitative RT-PCR analysis of miRNA in bovine solid tissues.
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 23 citations in OpenAlex.
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- CREB-dependent LPA-induced signaling initiates a pro-fibrotic feedback loop between small airway basal cells and fibroblasts.Respiratory research · 2021Article
- Different miRNA contents between mammary epithelial cells and milk fat globules: a random or a targeted process?Molecular biology reports · 2020Article
- Bta-miR-34b inhibits proliferation and promotes apoptosis via the MEK/ERK pathway by targeting MAP2K1 in bovine primary Sertoli cells.Journal of animal science · 2020Article
- MicroRNA-608 promotes apoptosis via BRD4 downregulation in pancreatic ductal adenocarcinoma.Oncology letters · 2020Article
- Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows.BMC genomics · 2019Article
- Characterization of new RNA polymerase III and RNA polymerase II transcriptional promoters in the Bovine Leukemia Virus genome.Scientific reports · 2016Article
- Annotation of the goat genome using next generation sequencing of microRNA expressed by the lactating mammary gland: comparison of three approaches.BMC genomics · 2015Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) has become the preferred technique for studying low-abundance RNA expression. Proper normalization is a critical but often underappreciated aspect of quantitative gene expression analysis; popular endogenous control genes are usually selected with little knowledge of their real suitability. To date, there are very few reports regarding the general validation of endogenous control genes for microRNA (miRNA) expression analysis in bovine tissue. In the present study, eight candidate reference genes (U6, 18S rRNA, GAPDH, ACTB, miR-191, miR-15a, miR-18a, let-7f) were tested for use as normalizers of bovine miRNA in RT-qPCR assays. Their selection was based on publicly available data concerning normalization, hierarchical clustering and sequencing. Three of the genes (miR-191, U6-1 and let-7f) were found to be highly consistent in their expression across eight different bovine solid tissues. It is commonly accepted that gene expression studies should be normalized using more than one endogenous control gene. Based on our results, we propose using the combined results for miR-191, U6-1 and let-7f as the endogenous control for normalization of miRNA levels in qRT-PCR analysis of diverse bovine tissues. This result could act a guideline for future work on bovine miRNA expression.
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