ArticleApplied and environmental microbiology2014
Design and validation of four new primers for next-generation sequencing to target the 18S rRNA genes of gastrointestinal ciliate protozoa.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
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- Diabetes and gut microbiome.Frontiers in microbiology · 2024Review
- Morphometric and Molecular Analysis of Five-SpineLife (Basel, Switzerland) · 2023Article
- Relationship Between Rumen Microbial Composition and Fibrolytic Isozyme Activity During the Biodegradation of Rice Straw Powder Using Rumen Fluid.Microbes and environments · 2023Article
- Genomic insights into the phylogeny and biomass-degrading enzymes of rumen ciliates.The ISME journal · 2022Article
- Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics.Microorganisms · 2021Article
- Ruminal Protozoal Populations of Angus Steers Differing in Feed Efficiency.Animals : an open access journal from MDPI · 2021Article
- Changes in Carbohydrate Composition in Fermented Total Mixed Ration and Its Effects onFrontiers in microbiology · 2021Article
- Seasonal and Nutrient Supplement Responses in Rumen Microbiota Structure and Metabolites of Tropical Rangeland Cattle.Microorganisms · 2020Article
- Extending Burk Dehority's Perspectives on the Role of Ciliate Protozoa in the Rumen.Frontiers in microbiology · 2020Review
- Assessment of biomass potentials of microalgal communities in open pond raceways using mass cultivation.PeerJ · 2020Article
- The community structure and microbial linkage of rumen protozoa and methanogens in response to the addition of tea seed saponins in the diet of beef cattle.Journal of animal science and biotechnology · 2020Article
- Temporal variation of eukaryotic community structures in UASB reactor treating domestic sewage as revealed by 18S rRNA gene sequencing.Scientific reports · 2019Article
- Genetic Analysis of Babesia Isolates from Cattle with Clinical Babesiosis in Sri Lanka.Journal of clinical microbiology · 2018Article
- Comparisons of bacterial and archaeal communities in the rumen and a dual-flow continuous culture fermentation system using amplicon sequencing.Journal of animal science · 2018Article
- Recent insights into the tick microbiome gained through next-generation sequencing.Parasites & vectors · 2018Review
- Addressing Global Ruminant Agricultural Challenges Through Understanding the Rumen Microbiome: Past, Present, and Future.Frontiers in microbiology · 2018Review
- Alteration of Rumen Bacteria and Protozoa Through Grazing Regime as a Tool to Enhance the Bioactive Fatty Acid Content of Bovine Milk.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Four new primers and one published primer were used to PCR amplify hypervariable regions within the protozoal 18S rRNA gene to determine which primer pair provided the best identification and statistical analysis. PCR amplicons of 394 to 498 bases were generated from three primer sets, sequenced using Roche 454 pyrosequencing with Titanium, and analyzed using the BLAST database (NCBI) and MOTHUR version 1.29. The protozoal diversity of rumen contents from moose in Alaska was assessed. In the present study, primer set 1, P-SSU-316F and GIC758R (amplicon of 482 bases), gave the best representation of diversity using BLAST classification, and the set amplified Entodinium simplex and Ostracodinium spp., which were not amplified by the other two primer sets. Primer set 2, GIC1080F and GIC1578R (amplicon of 498 bases), had similar BLAST results and a slightly higher percentage of sequences that were identified with a higher sequence identity. Primer sets 1 and 2 are recommended for use in ruminants. However, primer set 1 may be inadequate to determine protozoal diversity in nonruminants. The amplicons created by primer set 1 were indistinguishable for certain species within the genera Bandia, Blepharocorys, Polycosta, and Tetratoxum and between Hemiprorodon gymnoprosthium and Prorodonopsis coli, none of which are normally found in the rumen.
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