ArticleApplied and environmental microbiology2014
A Pseudomonas putida strain genetically engineered for 1,2,3-trichloropropane bioremediation.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 54 citations in OpenAlex.
- Isolation and Characterization of a Crude Oil-Tolerant Obligate Halophilic Bacterium from the Great Salt Lake of the United States of America.Microorganisms · 2025Article
- Microbes' role in environmental pollution and remediation: a bioeconomy focus approach.AIMS microbiology · 2024Review
- Mirror proteorhodopsins.Communications chemistry · 2023Article
- Biodegradation of aromatic pollutants meets synthetic biology.Synthetic and systems biotechnology · 2021Article
- CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants.Synthetic and systems biotechnology · 2020Review
- Review
- A Robust CRISPR Interference Gene Repression System in Pseudomonas.Journal of bacteriology · 2018Article
- Inactivation of 4-Oxalocrotonate Tautomerase by 5-Halo-2-hydroxy-2,4-pentadienoates.Biochemistry · 2018Article
- Microbial Synthesis and Transformation of Inorganic and Organic Chlorine Compounds.Frontiers in microbiology · 2018Review
- Combinatorial metabolic engineering of Pseudomonas putida KT2440 for efficient mineralization of 1,2,3-trichloropropane.Scientific reports · 2017Article
- Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway.Microbial cell factories · 2015Article
- Efflux systems in bacteria and their metabolic engineering applications.Applied microbiology and biotechnology · 2015Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1,2,3-Trichloropropane (TCP) is a toxic compound that is recalcitrant to biodegradation in the environment. Attempts to isolate TCP-degrading organisms using enrichment cultivation have failed. A potential biodegradation pathway starts with hydrolytic dehalogenation to 2,3-dichloro-1-propanol (DCP), followed by oxidative metabolism. To obtain a practically applicable TCP-degrading organism, we introduced an engineered haloalkane dehalogenase with improved TCP degradation activity into the DCP-degrading bacterium Pseudomonas putida MC4. For this purpose, the dehalogenase gene (dhaA31) was cloned behind the constitutive dhlA promoter and was introduced into the genome of strain MC4 using a transposon delivery system. The transposon-located antibiotic resistance marker was subsequently removed using a resolvase step. Growth of the resulting engineered bacterium, P. putida MC4-5222, on TCP was indeed observed, and all organic chlorine was released as chloride. A packed-bed reactor with immobilized cells of strain MC4-5222 degraded >95% of influent TCP (0.33 mM) under continuous-flow conditions, with stoichiometric release of inorganic chloride. The results demonstrate the successful use of a laboratory-evolved dehalogenase and genetic engineering to produce an effective, plasmid-free, and stable whole-cell biocatalyst for the aerobic bioremediation of a recalcitrant chlorinated hydrocarbon.
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