ArticleMolecular biotechnology2014
Improving the efficiency of transposon mutagenesis in Salmonella enteritidis by overcoming host-restriction barriers.
Article in Molecular biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- UsingACS synthetic biology · 2025Article
- Genetic Determinants in Salmonella enterica Serotype Typhimurium Required for OvercomingMicrobiology spectrum · 2021Article
- Identification of Genes Involved in Antifungal Activity ofPathogens (Basel, Switzerland) · 2020Article
- Toward a genetic system in the marine cyanobacteriumAccess microbiology · 2020Article
- MexAB-OprM Efflux Pump ofFrontiers in microbiology · 2019Article
- Large-Scale Analysis of theFrontiers in microbiology · 2019Article
- Essential genome of Campylobacter jejuni.BMC genomics · 2017Article
- Global Screening ofFrontiers in microbiology · 2017Article
- Genome modifications and cloning using a conjugally transferable recombineering system.Biotechnology reports (Amsterdam, Netherlands) · 2015Article
- Heat Shock-Enhanced Conjugation Efficiency in Standard Campylobacter jejuni Strains.Applied and environmental microbiology · 2015Article
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Authors and funding
5 authors.
Funding
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Abstract
Transposon mutagenesis using transposome complex is a powerful method for functional genomics analysis in diverse bacteria by creating a large number of random mutants to prepare a genome-saturating mutant library. However, strong host restriction barriers can lead to limitations with species- or strain-specific restriction-modification systems. The purpose of this study was to enhance the transposon mutagenesis efficiency of Salmonella Enteritidis to generate a larger number of random insertion mutants. Host-adapted Tn5 DNA was used to form a transposome complex, and this simple approach significantly and consistently improved the efficiency of transposon mutagenesis, resulting in a 46-fold increase in the efficiency as compared to non-adapted transposon DNA fragments. Random nature of Tn5 insertions was confirmed by high-throughput sequencing of the Tn5-junction sequences. The result based on S. Enteritidis in this study should find broad applications in preparing a comprehensive mutant library of other species using transposome complex.
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