ArticleExperimental eye research2014
Assessment of anti-scarring therapies in ex vivo organ cultured rabbit corneas.
Article in Experimental eye research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Nanomedicine in Ophthalmology: From Bench to Bedside.Journal of clinical medicine · 2024Review
- Regenerative Therapy for Corneal Scarring Disorders.Biomedicines · 2024Review
- Review
- NBL1 Reduces Corneal Fibrosis and Scar Formation after Wounding.Biomolecules · 2023Article
- Establishment of a Robust and Simple Corneal Organ Culture Model to Monitor Wound Healing.Journal of clinical medicine · 2021Article
- Corneal stromal wound healing: Major regulators and therapeutic targets.The ocular surface · 2021Review
- Article
- Quercetin suppresses glomerulosclerosis and TGF‑β signaling in a rat model.Molecular medicine reports · 2019Article
- Tissue-derived microparticles reduce inflammation and fibrosis in cornea wounds.Acta biomaterialia · 2019Article
- Corneal chemical burn treatment through a delivery system consisting of TGF-βDrug delivery and translational research · 2018Article
- Apratyramide, a Marine-Derived Peptidic Stimulator of VEGF-A and Other Growth Factors with Potential Application in Wound Healing.ACS chemical biology · 2018Article
- Tranilast inhibits TGF-β-induced collagen gel contraction mediated by human corneal fibroblasts.International journal of ophthalmology · 2018Article
- Analysis of dermal fibroblasts isolated from neonatal and child cleft lip and adult skin: Developmental implications on reconstructive surgery.International journal of molecular medicine · 2017Article
- Myofibroblast transdifferentiation: The dark force in ocular wound healing and fibrosis.Progress in retinal and eye research · 2017Review
- Development of wound healing models to study TGFβ3's effect on SMA.Experimental eye research · 2017Article
- Ex vivo rabbit and human corneas as models for bacterial and fungal keratitis.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2017Article
- Connective tissue growth factor is not necessary for haze formation in excimer laser wounded mouse corneas.PloS one · 2017Article
- Article
- Progress in corneal wound healing.Progress in retinal and eye research · 2015Review
- Human Serum Eye Drops in Eye Alterations: An Insight and a Critical Analysis.Journal of ophthalmology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The effects of a triple combination of siRNAs targeting key scarring genes were assessed using an ex vivo organ culture model of excimer ablated rabbit corneas. The central 6 mm diameter region of fresh rabbit globes was ablated to a depth of 155 microns with an excimer laser. Corneas were excised, cultured at the air-liquid interface in defined culture medium supplemented with transforming growth factor beta 1 (TGFB1), and treated with either 1% prednisolone acetate or with 22.5 μM cationic nanoparticles complexed with a triple combination of siRNAs (NP-siRNA) targeting TGFB1, TGFB Receptor (TGFBR2) and connective tissue growth factor (CTGF). Scar formation was measured using image analysis of digital images and levels of smooth muscle actin (SMA) were assessed in ablated region of corneas using qRT-PCR and immunostaining. Ex vivo cultured corneas developed intense haze-like scar in the wounded areas and levels of mRNAs for pro-fibrotic genes were significantly elevated 3-8 fold in wounded tissue compared to unablated corneas. Treatment with NP-siRNA or steroid significantly reduced quantitative haze levels by 55% and 68%, respectively, and reduced SMA mRNA and immunohistostaining. This ex vivo corneal culture system reproduced key molecular patterns of corneal scarring and haze formation generated in rabbits. Treatment with NP-siRNAs targeting key scarring genes or an anti-inflammatory steroid reduced corneal haze and SMA mRNA and protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.