ReviewBioMed research international2014
Application of PCR-ELISA in molecular diagnosis.
Review in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nucleic Acid Amplification Tests forPathogens (Basel, Switzerland) · 2026Pooled it
- Strategy for Improving the Diagnostic Efficacy of Bovine Tuberculosis Using a Novel PCRVeterinary research communications · 2026Article
- Review
- Analytical Performance of Nanobody-Based Immunoassay and Immunosensing Platforms for Bacteria and Toxin Detection: A Systematic Review.Antibodies (Basel, Switzerland) · 2026Review
- Rapid and simple on-site salmonella detection in food via direct sample loading using a lipopolysaccharide-imprinted polymer.Journal of nanobiotechnology · 2025Article
- High-throughput homogenous assay for the direct detection of Listeria monocytogenes DNA.Scientific reports · 2024Article
- Simultaneous detection of seven bacterial pathogens transmitted by flies using the reverse line blot hybridization assay.Parasites & vectors · 2024Article
- Recent Developments in Lateral Flow Assays forPathogens (Basel, Switzerland) · 2023Review
- Mucormycosis diagnosis revisited: Current and emerging diagnostic methodologies for the invasive fungal infection (Review).Experimental and therapeutic medicine · 2023Review
- Immunosensors-The Future of Pathogen Real-Time Detection.Sensors (Basel, Switzerland) · 2022Review
- Recent trends and developments of PCR-based methods for the detection of food-borneJournal of food science and technology · 2022Review
- Polyaniline-Pectin nanoparticles immobilized paper based colorimetric sensor for detection ofCurrent research in food science · 2022Article
- Advancement inBiosensors · 2021Review
- Nanodiagnostics in leishmaniasis: A new frontiers for early elimination.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2021Review
- Recent trends in rapid diagnostic techniques for dermatophytosis.International journal of veterinary science and medicine · 2020Review
- Biosensors and Diagnostics for Fungal Detection.Journal of fungi (Basel, Switzerland) · 2020Review
- Sensitive detection of the ISRSC advances · 2018Article
- Molecular Diagnosis of Visceral Leishmaniasis.Molecular diagnosis & therapy · 2018Review
- Molecular biological tools applied for identification of mastitis causing pathogens.International journal of veterinary science and medicine · 2017Review
- Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection byJournal of tropical medicine · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) is an immunodetection method that can quantify PCR product directly after immobilization of biotinylated DNA on a microplate. This method, which detects nucleic acid instead of protein, is a much more sensitive method compared to conventional PCR method, with shorter analytical time and lower detection limit. Its high specificity and sensitivity, together with its semiquantitative ability, give it a huge potential to serve as a powerful detection tool in various industries such as medical, veterinary, and agricultural industries. With the recent advances in PCR-ELISA, it is envisaged that the assay is more widely recognized for its fast and sensitive detection limit which could improve overall diagnostic time and quality.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.