ArticleThe Journal of cell biology1989
Characterization of synapsin I fragments produced by cysteine-specific cleavage: a study of their interactions with F-actin.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 117 citations in OpenAlex.
- Condensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization.The EMBO journal · 2025Article
- Inhibition of hippocampal mossy fiber plasticity and episodic memory by human Aβ oligomers is prevented by enhancing cAMP signaling in Alzheimer's mice.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- Condensates of synaptic vesicles and synapsin are molecular beacons for actin sequestering and polymerization.bioRxiv : the preprint server for biology · 2025Article
- Crosstalk of Synapsin1 palmitoylation and phosphorylation controls the dynamicity of synaptic vesicles in neurons.Cell death & disease · 2022Article
- Non-canonical soluble amyloid-beta aggregates and plaque buffering: controversies and future directions for target discovery in Alzheimer's disease.Alzheimer's research & therapy · 2017Review
- The Presynaptic Microtubule Cytoskeleton in Physiological and Pathological Conditions: Lessons from Drosophila Fragile X Syndrome and Hereditary Spastic Paraplegias.Frontiers in molecular neuroscience · 2016Review
- Functional distribution of synapsin I in human sperm.FEBS open bio · 2015Article
- Article
- Binding of protein kinase inhibitors to synapsin I inferred from pair-wise binding site similarity measurements.PloS one · 2010Article
- A tris (2-carboxyethyl) phosphine (TCEP) related cleavage on cysteine-containing proteins.Journal of the American Society for Mass Spectrometry · 2010Article
- Structural domains involved in the regulation of transmitter release by synapsins.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Colocalization of synapsin and actin during synaptic vesicle recycling.The Journal of cell biology · 2003Article
- Identification of synapsin I peptides that insert into lipid membranes.The Biochemical journal · 2001Article
- Synapsin III: developmental expression, subcellular localization, and role in axon formation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Synapsins as regulators of neurotransmitter release.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1999Review
- Kinetic analysis of the phosphorylation-dependent interactions of synapsin I with rat brain synaptic vesicles.The Journal of physiology · 1997Article
- Synapsin I interacts with c-Src and stimulates its tyrosine kinase activity.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Identification, expression, and crystallization of the protease-resistant conserved domain of synapsin I.Protein science : a publication of the Protein Society · 1997Article
- Structural analysis of human immunodeficiency virus type 1 Gag protein interactions, using cysteine-specific reagents.Journal of virology · 1996Article
- Synapsin I deficiency results in the structural change in the presynaptic terminals in the murine nervous system.The Journal of cell biology · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Synapsin I is a neuron-specific phosphoprotein that is concentrated in the presynaptic nerve terminal in association with the cytoplasmic surface of synaptic vesicles. It has been demonstrated to bundle F-actin in a phosphorylation-dependent manner in vitro, a property consistent with its proposed role in linking synaptic vesicles to the cytoskeleton and its involvement in the regulation of neurotransmitter release. Synapsin I is composed of two distinct domains, a COOH terminal, collagenase-sensitive, hydrophilic, and strongly basic tail region, and an NH2 terminal, collagenase-resistant head region relatively rich in hydrophobic amino acids. To elucidate the structural basis for the interactions between synapsin I and F-actin and how it relates to other characteristics of synapsin I, we have performed a structure-function analysis of fragments of synapsin I produced by cysteine-specific cleavage with 2-nitro-5-thiocyanobenzoic acid. The fragments were identified and aligned with the parent molecule using the deduced primary structure of synapsin I and the known phosphorylation sites as markers. We have purified these fragments and examined their interactions with F-actin. Two distinct fragments, a 29-kD NH2-terminal fragment and a 15-kD middle fragment, were shown to contain F-actin binding sites. A 51/54-kD middle/tail fragment retained the F-actin binding and bundling activity of synapsin I, but the isolated tail fragment did not retain either activity. In contrast to phosphorylation of sites two and three in intact synapsin I, which abolishes F-actin bundling activity, phosphorylation of these sites in the middle/tail fragment failed to abolish this activity. In conclusion, three domains of synapsin I appear to be involved in F-actin binding and bundling.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.