ArticleInvestigative ophthalmology & visual science2014
Endogenous fluorophores enable two-photon imaging of the primate eye.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 32 citations in OpenAlex.
- Structural changes in the crystalline lens as a function of the postmortem interval assessed with two-photon imaging microscopy.Biomedical optics express · 2024Article
- Lipofuscin, Its Origin, Properties, and Contribution to Retinal Fluorescence as a Potential Biomarker of Oxidative Damage to the Retina.Antioxidants (Basel, Switzerland) · 2023Review
- From mouse to human: Accessing the biochemistry of vision in vivo by two-photon excitation.Progress in retinal and eye research · 2023Review
- Fluorescence Angiography with Dual Fluorescence for the Early Detection and Longitudinal Quantitation of Vascular Leakage in Retinopathy.Biomedicines · 2023Article
- Stability of retinol in liposomes as measured by fluorescence lifetime spectroscopy and FLIM.BBA advances · 2023Article
- Two-photon excitation fluorescence in ophthalmology: safety and improved imaging for functional diagnostics.Frontiers in medicine · 2023Review
- In vivo imaging of the human eye using a 2-photon-excited fluorescence scanning laser ophthalmoscope.The Journal of clinical investigation · 2022Article
- Adaptive optics two-photon excited fluorescence lifetime imaging ophthalmoscopy of photoreceptors and retinal pigment epithelium in the living non-human primate eye.Biomedical optics express · 2022Article
- Retinal Organoids Long-Term Functional Characterization Using Two-Photon Fluorescence Lifetime and Hyperspectral Microscopy.Frontiers in cellular neuroscience · 2021Article
- Noninvasive two-photon optical biopsy of retinal fluorophores.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Characterization of Retinol Stabilized in Phosphatidylcholine Vesicles with and without Antioxidants.ACS omega · 2020Article
- Multistate Multiconfiguration Quantum Chemical Computation of the Two-Photon Absorption Spectra of Bovine Rhodopsin.The journal of physical chemistry letters · 2019Article
- Sensitivity of Mammalian Cone Photoreceptors to Infrared Light.Neuroscience · 2019Article
- Retinoid isomerase inhibitors impair but do not block mammalian cone photoreceptor function.The Journal of general physiology · 2018Article
- Imaging individual neurons in the retinal ganglion cell layer of the living eye.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Formation and Clearance of All-Trans-Retinol in Rods Investigated in the Living Primate Eye With Two-Photon Ophthalmoscopy.Investigative ophthalmology & visual science · 2017Article
- Image registration and averaging of low laser power two-photon fluorescence images of mouse retina.Biomedical optics express · 2016Article
- In vivo multiphoton tomography and fluorescence lifetime imaging of human brain tumor tissue.Journal of neuro-oncology · 2016Article
- Two-Photon Autofluorescence Imaging Reveals Cellular Structures Throughout the Retina of the Living Primate Eye.Investigative ophthalmology & visual science · 2016Article
- In Vivo Two-Photon Fluorescence Kinetics of Primate Rods and Cones.Investigative ophthalmology & visual science · 2016Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
purposeNoninvasive two-photon imaging of a living mammalian eye can reveal details of molecular processes in the retina and RPE. Retinyl esters and all-trans-retinal condensation products are two types of retinoid fluorophores present in these tissues. We measured the content of these two types of retinoids in monkey and human eyes to validate the potential of two-photon imaging for monitoring retinoid changes in human eyes.
methodsTwo-photon microscopy (TPM) was used to visualize excised retina from monkey eyes. Retinoid composition and content in human and monkey eyes were quantified by HPLC and mass spectrometry (MS).
resultsClear images of inner and outer segments of rods and cones were obtained in primate eyes at different eccentricities. Fluorescence spectra from outer segments revealed a maximum emission at 480 nm indicative of retinols and their esters. In cynomolgus monkey and human retinal extracts, retinyl esters existed predominantly in the 11-cis configuration along with notable levels of 11-cis-retinol, a characteristic of cone-enriched retinas. Average amounts of di-retinoid-pyridinium-ethanolamine (A2E) in primate and human eyes were 160 and 225 pmol/eye, respectively.
conclusionsThese data show that human retina contains sufficient amounts of retinoids for two-photon excitation imaging. Greater amounts of 11-cis-retinyl esters relative to rodent retinas contribute to the fluorescence signal from both monkey and human eyes. These observations indicate that TPM imaging found effective in mice could detect early age- and disease-related changes in human retina.
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