ArticleBiomolecules2013
A sensitive DNA enzyme-based fluorescent assay for bacterial detection.
Article in Biomolecules, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Enhanced Detection ofBiosensors · 2024Article
- DNAzyme-based faithful probing and pulldown to identify candidate biomarkers of low abundance.Nature chemistry · 2024Article
- Engineering a Ligase Binding DNA Aptamer into a Templating DNA Scaffold to Guide the Selective Synthesis of Circular DNAzymes and DNA Aptamers.Journal of the American Chemical Society · 2023Article
- RNA-cleaving DNAzymes as a diagnostic and therapeutic agent against antimicrobial resistant bacteria.Current genetics · 2022Review
- DigitalBiosensors · 2022Article
- Advances in the Application of Nanomaterials as Treatments for Bacterial Infectious Diseases.Pharmaceutics · 2021Review
- Integrating programmable DNAzymes with electrical readout for rapid and culture-free bacterial detection using a handheld platform.Nature chemistry · 2021Article
- Biosensing with DNAzymes.Chemical Society reviews · 2021Review
- Selection and applications of functional nucleic acids for infectious disease detection and prevention.Analytical and bioanalytical chemistry · 2021Review
- Detection ofFrontiers in microbiology · 2021Article
- Graphene biosensors for bacterial and viral pathogens.Biosensors & bioelectronics · 2020Review
- In vitro Selection of Chemically Modified DNAzymes.ChemistryOpen · 2020Review
- Microbial Infections as a Trigger for Acute-on-Chronic Liver Failure: A Review.Medical science monitor : international medical journal of experimental and clinical research · 2019Review
- A Simple DNAzyme-Based Fluorescent Assay for Klebsiella pneumoniae.Chembiochem : a European journal of chemical biology · 2019Article
- A Printed Multicomponent Paper Sensor for Bacterial Detection.Scientific reports · 2017Article
- The Optimization and Characterization of an RNA-Cleaving Fluorogenic DNAzyme Probe for MDA-MB-231 Cell Detection.Sensors (Basel, Switzerland) · 2017Article
- Theranostic DNAzymes.Theranostics · 2017Review
- Integrating Deoxyribozymes into Colorimetric Sensing Platforms.Sensors (Basel, Switzerland) · 2016Review
- Colorimetric Detection of Bacteria Using Litmus Test.Journal of visualized experiments : JoVE · 2016Article
- Programming a topologically constrained DNA nanostructure into a sensor.Nature communications · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial detection plays an important role in protecting public health and safety, and thus, substantial research efforts have been directed at developing bacterial sensing methods that are sensitive, specific, inexpensive, and easy to use. We have recently reported a novel "mix-and-read" assay where a fluorogenic DNAzyme probe was used to detect model bacterium E. coli. In this work, we carried out a series of optimization experiments in order to improve the performance of this assay. The optimized assay can achieve a detection limit of 1000 colony-forming units (CFU) without a culturing step and is able to detect 1 CFU following as short as 4 h of bacterial culturing in a growth medium. Overall, our effort has led to the development of a highly sensitive and easy-to-use fluorescent bacterial detection assay that employs a catalytic DNA.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.