ArticleScientific reports2014
Spectral and structural comparison between bright and dim green fluorescent proteins in Amphioxus.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Fluorescent proteins generate a genetic color polymorphism and counteract oxidative stress in intertidal sea anemones.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Post-Golgi carriers, not lysosomes, confer lysosomal properties to pre-degradative organelles in normal and dystrophic axons.Cell reports · 2021Article
- Hederagenin potentiated cisplatin- and paclitaxel-mediated cytotoxicity by impairing autophagy in lung cancer cells.Cell death & disease · 2020Article
- Association of Fluorescent Protein Pairs and Its Significant Impact on Fluorescence and Energy Transfer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Structural Factors Enabling Successful GFP-Like Proteins with Alanine as the Third Chromophore-Forming Residue.Journal of molecular biology · 2019Article
- Fluorescent Proteins for Investigating Biological Events in Acidic Environments.International journal of molecular sciences · 2018Review
- Photophysical Behavior of mNeonGreen, an Evolutionarily Distant Green Fluorescent Protein.Biophysical journal · 2018Article
- Nanoscale gizmos - the novel fluorescent probes for monitoring protein activity.Biochemical engineering journal · 2018Review
- Multi-color fluorescent reporter dengue viruses with improved stability for analysis of a multi-virus infection.PloS one · 2018Article
- ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement.Scientific reports · 2017Article
- Molecular evolution of versatile derivatives from a GFP-like protein in the marine copepod Chiridius poppei.PloS one · 2017Article
- The evolution of genes encoding for green fluorescent proteins: insights from cephalochordates (amphioxus).Scientific reports · 2016Article
- GFP-complementation assay to detect functional CPP and protein delivery into living cells.Scientific reports · 2015Article
- An optical biosensor from green fluorescent Escherichia coli for the evaluation of single and combined heavy metal toxicities.Sensors (Basel, Switzerland) · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cephalochordate Amphioxus naturally co-expresses fluorescent proteins (FPs) with different brightness, which thus offers the rare opportunity to identify FP molecular feature/s that are associated with greater/lower intensity of fluorescence. Here, we describe the spectral and structural characteristics of green FP (bfloGFPa1) with perfect (100%) quantum efficiency yielding to unprecedentedly-high brightness, and compare them to those of co-expressed bfloGFPc1 showing extremely-dim brightness due to low (0.1%) quantum efficiency. This direct comparison of structure-function relationship indicated that in the bright bfloGFPa1, a Tyrosine (Tyr159) promotes a ring flipping of a Tryptophan (Trp157) that in turn allows a cis-trans transformation of a Proline (Pro55). Consequently, the FP chromophore is pushed up, which comes with a slight tilt and increased stability. FPs are continuously engineered for improved biochemical and/or photonic properties, and this study provides new insight to the challenge of establishing a clear mechanistic understanding between chromophore structural environment and brightness level.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.