Evidence map›Paper›PMID 24968758›Full record

ArticleUltrasound in medicine & biology2014

High-frequency ultrasonic imaging of growth and development in manufactured engineered oral mucosal tissue surfaces.

Frank Winterroth, Hiroko Kato, Shiuhyang Kuo, Stephen E Feinberg, Scott J Hollister, J Brian Fowlkes, Kyle W Hollman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ultrasound in medicine & biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Frank WinterrothDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA. Electronic address: Fwinterr@umich.edu.
Hiroko KatoDepartment of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA; Department of Oral Anatomy, Course for Oral Life Science, Niigata University Postgraduate School of Medical and Dental Sciences, Niigata, Japan.
Shiuhyang KuoDepartment of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA.
Stephen E FeinbergDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, Michigan, USA.
Scott J HollisterDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Department of Surgery, University of Michigan, Ann Arbor, Michigan, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
J Brian FowlkesDepartment of Radiology, University of Michigan, Ann Arbor, Michigan, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Kyle W HollmanDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Sound Sight Research, Livonia, Michigan, USA.

Funding

VIVARIUM MODULEP30EY007003 · NEI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI David Antonetti · 1987 to 2026
$17.6M
Non-invasive Assessment of Tissue Engineered Human Oral MucosaR01DE019431 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FEINBERG, STEPHEN ELLIOTT, MYCEK, MARY-ANN · 2009 to 2012
$3.0M
EX VIVO DEVELOPMENT OF TRANSFECTED ORAL MUCOSAL GRAFTSR01DE013417 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FEINBERG, STEPHEN ELLIOTT · 2000 to 2009
$2.9M
High-frequency 3-dimensional ultrasound elasticity imaging of the corneaR21EY018727 · NEI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HOLLMAN, KYLE W · 2008 to 2009
$390k
E75 IN THE DEVELOPING DROSOPHILA EYEF31EY007003 · NEI · YALE UNIVERSITY · PI SHILTON, ALYCIA N · 1999 to 2001
$14k
NEI NIH HHS EY007003NEI NIH HHS F31 EY007003NEI NIH HHS P30 EY007003NEI NIH HHS R21 EY018727NIDCR NIH HHS R01 DE013417NIDCR NIH HHS R01 DE019431NIDCR NIH HHS R01 DE13417
6 · The paper itself

Abstract

This study uses high-resolution ultrasound to examine the growth and development of engineered oral mucosal tissues manufactured under aseptic conditions. The specimens are a commercially available natural tissue scaffold, AlloDerm, and oral keratinocytes seeded onto AlloDerm to form an ex vivo-produced oral mucosal equivalent (EVPOME) suitable for intra-oral grafting. The seeded cells produce a keratinized protective upper layer that smooths out any remaining surface irregularities on the underlying AlloDerm. Two-dimensional acoustic imaging of unseeded AlloDerm and developing EVPOMEs was performed on each day of their growth and development, each tissue specimen being imaged under aseptic conditions (total time from seeding to maturation: 11 d). Ultrasonic monitoring offers us the ability to determine the constituents of the EVPOME that are responsible for changes in its mechanical behavior during the manufacturing process. Ultrasonic monitoring affords us an opportunity to non-invasively assess, in real time, tissue-engineered constructs before release for use in patient care.

Indexed as

CollagenTissue ScaffoldsCells, CulturedHumansMouth MucosaTissue EngineeringUltrasonographyAllodermCollagenAcoustic elasticity microscopyCell spreadingImage analysisKeratinocyteNon-invasive assessmentOral mucosaTissue engineeringUltrasound

Identifiers

PMID24968758
PMCPMC4130788

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.