ArticleClinica chimica acta; international journal of clinical chemistry2014
A high-throughput robotic sample preparation system and HPLC-MS/MS for measuring urinary anatabine, anabasine, nicotine and major nicotine metabolites.
Article in Clinica chimica acta; international journal of clinical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The trial behind it
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Who cites it
46 citing papers in PubMed.
- The Impact of Exclusive Use of Very Low Nicotine Cigarettes on Compensatory Smoking: An Inpatient Crossover Clinical Trial.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2020Trial
- Nicotine and tobacco transitions among US youth using electronic nicotine delivery systems (ENDS): Associations with frequency, nicotine dependence, and biomarkers of exposure.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2026Article
- Altered Regional Brain Activity Underlying the Higher Postoperative Analgesic Requirements in Abstinent Smokers: A Prospective Cohort Study.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- A Streamlined Automated Liquid Handling and 96-Well Solid-Phase Extraction for Single Low-Volume Urine Aliquot Multiclass Biomarker Analysis in Human Biomonitoring.Environmental chemistry and ecotoxicology · 2026Article
- Tobacco Smoke Exposure Biomarker Profiles and Healthcare Utilization Patterns Among U.S. Children.Toxics · 2025Article
- Electronic Nicotine Delivery Systems Use and Nicotine Exposure Among Adults Who Use ENDS Alone or With Cigarettes: The Population Assessment of Tobacco and Health Study 2013-2019.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2025Article
- Nicotine and Toxicant Exposure among Individuals using both Combustible Cigarettes and E-cigarettes Based on Level of Product Use.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2025Article
- Biomarkers of Exposure to Nicotine and Selected Toxicants in Individuals Who Use Alternative Tobacco Products Sold in Japan and Canada from 2018 to 2019.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2025Article
- Computer Vision-Assisted Robotized Sampling of Volatile Organic Compounds.Analytical chemistry · 2024Article
- Influence of Half-life and Smoking/Nonsmoking Ratio on Biomarker Consistency between Waves 1 and 2 of the Population Assessment of Tobacco and Health Study.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2024Article
- Quantitation of Ten Urinary Nicotine Metabolites, Including 4-Hydroxy-4-(3-pyridyl) Butanoic Acid, a Product of Nicotine 2'-Oxidation, and CYP2A6 Activity in Japanese Americans, Native Hawaiians, and Whites.Chemical research in toxicology · 2023Article
- Distinguishing Exposure to Secondhand and Thirdhand Tobacco Smoke among U.S. Children Using Machine Learning: NHANES 2013-2016.Environmental science & technology · 2023Article
- Simultaneous quantification of urinary tobacco and marijuana metabolites using solid-supported liquid-liquid extraction coupled with liquid chromatography tandem mass spectrometry.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2022Article
- Anabasine and Anatabine Exposure Attributable to Cigarette Smoking: National Health and Nutrition Examination Survey (NHANES) 2013-2014.International journal of environmental research and public health · 2022Article
- Urinary Nicotine Metabolites and Self-Reported Tobacco Use Among Adults in the Population Assessment of Tobacco and Health (PATH) Study, 2013-2014.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2022Article
- Nicotine Exposure in the U.S. Population: Total Urinary Nicotine Biomarkers in NHANES 2015-2016.International journal of environmental research and public health · 2022Article
- Application of HPLC-QQQ-MS/MS and New RP-HPLC-DAD System Utilizing the Chaotropic Effect for Determination of Nicotine and Its Major Metabolites Cotinine, andMolecules (Basel, Switzerland) · 2022Article
- Minor Tobacco Alkaloids as Biomarkers to Distinguish Combusted Tobacco Use From Electronic Nicotine Delivery Systems Use. Two New Analytical Methods.Frontiers in chemistry · 2022Article
- Article
- Sexual identity differences in biomarkers of tobacco exposure among women in a national sample.Cancer epidemiology · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundMost sample preparation methods characteristically involve intensive and repetitive labor, which is inefficient when preparing large numbers of samples from population-scale studies.
methodsThis study presents a robotic system designed to meet the sampling requirements for large population-scale studies. Using this robotic system, we developed and validated a method to simultaneously measure urinary anatabine, anabasine, nicotine and seven major nicotine metabolites: 4-Hydroxy-4-(3-pyridyl)butanoic acid, cotinine-N-oxide, nicotine-N-oxide, trans-3'-hydroxycotinine, norcotinine, cotinine and nornicotine. We analyzed robotically prepared samples using high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry in positive electrospray ionization mode using scheduled multiple reaction monitoring (sMRM) with a total runtime of 8.5 min.
resultsThe optimized procedure was able to deliver linear analyte responses over a broad range of concentrations. Responses of urine-based calibrators delivered coefficients of determination (R(2)) of >0.995. Sample preparation recovery was generally higher than 80%. The robotic system was able to prepare four 96-well plate (384 urine samples) per day, and the overall method afforded an accuracy range of 92-115%, and an imprecision of <15.0% on average.
conclusionsThe validation results demonstrate that the method is accurate, precise, sensitive, robust, and most significantly labor-saving for sample preparation, making it efficient and practical for routine measurements in large population-scale studies such as the National Health and Nutrition Examination Survey (NHANES) and the Population Assessment of Tobacco and Health (PATH) study.
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