ArticleInternational journal of molecular sciences2014
Insights on antioxidant assays for biological samples based on the reduction of copper complexes-the importance of analytical conditions.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Olive Oil Waste as a Source of Functional Food Ingredients: Assessing Polyphenolic Content and Antioxidant Activity in Olive Leaves.Foods (Basel, Switzerland) · 2024Article
- Liver Oxidative Status, Serum Lipids Levels after Bariatric Surgery and High-Fat, High-Sugar Diet in Animal Model of Induced Obesity.International journal of molecular sciences · 2023Article
- Total Antioxidant Capacity: Biochemical Aspects and Clinical Significance.International journal of molecular sciences · 2023Review
- Synergetic Effect of Tumor Treating Fields and Zinc Oxide Nanoparticles on Cell Apoptosis and Genotoxicity of Three Different Human Cancer Cell Lines.Molecules (Basel, Switzerland) · 2022Article
- Impact of coffee preparation on total phenolic content in brewed coffee extracts and their contribution to the body's antioxidant status.Food science and biotechnology · 2022Article
- Future Perspectives in Oxidative Stress in Trisomy 13 and 18 Evaluation.Journal of clinical medicine · 2022Review
- Nanovesicles from Organic Agriculture-Derived Fruits and Vegetables: Characterization and Functional Antioxidant Content.International journal of molecular sciences · 2021Article
- Analytical Methods Used in Determining Antioxidant Activity: A Review.International journal of molecular sciences · 2021Review
- Bladder cancer: total antioxidant capacity and pharmacotherapy with vitamin-E.International urology and nephrology · 2020Article
- Effect on Adipose Tissue of Diabetic Mice Supplemented with n-3 Fatty Acids Extracted from Microalgae.Endocrine, metabolic & immune disorders drug targets · 2020Article
- Metal Oxide Nanoparticle Based Electrochemical Sensor for Total Antioxidant Capacity (TAC) Detection in Wine Samples.Biosensors · 2018Article
- Antioxidant efficacy of chitosan/graphene functionalized superparamagnetic iron oxide nanoparticles.Journal of materials science. Materials in medicine · 2018Article
- Dietary Phytochemicals Promote Health by Enhancing Antioxidant Defence in a Pig Model.Nutrients · 2017Article
- Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update.BMC veterinary research · 2016Review
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4 authors.
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Abstract
Total antioxidant capacity assays are recognized as instrumental to establish antioxidant status of biological samples, however the varying experimental conditions result in conclusions that may not be transposable to other settings. After selection of the complexing agent, reagent addition order, buffer type and concentration, copper reducing assays were adapted to a high-throughput scheme and validated using model biological antioxidant compounds of ascorbic acid, Trolox (a soluble analogue of vitamin E), uric acid and glutathione. A critical comparison was made based on real samples including NIST-909c human serum certified sample, and five study samples. The validated method provided linear range up to 100 µM Trolox, (limit of detection 2.3 µM; limit of quantification 7.7 µM) with recovery results above 85% and precision <5%. The validated developed method with an increased sensitivity is a sound choice for assessment of TAC in serum samples.
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