ArticleJournal of immunological methods2014
Toward development of a comprehensive external quality assurance program for polyfunctional intracellular cytokine staining assays.
Article in Journal of immunological methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Strengthening the Application of the DAIDS GCLP Guidelines: The Implementation of an Integrated Laboratory Oversight Framework.AIDS research and human retroviruses · 2024Article
- Improving Reliability of Immunological Assays by Defining Minimal Criteria for Cell Fitness.ImmunoHorizons · 2024Article
- TEAM: A MULTIPLE TESTING ALGORITHM ON THE AGGREGATION TREE FOR FLOW CYTOMETRY ANALYSIS.The annals of applied statistics · 2023Article
- Achieving intracellular cytokine staining assay concordance on two continents to assess HIV vaccine-induced T-cell responses.Journal of leukocyte biology · 2022Article
- FlowKit: A Python Toolkit for Integrated Manual and Automated Cytometry Analysis Workflows.Frontiers in immunology · 2021Article
- A Cytometrist's Guide to Coordinating and Performing Effective COVID-19 Research.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Immune Monitoring for CMV in Transplantation.Current infectious disease reports · 2018Review
- Variability of the IFN-γ ELISpot assay in the context of proficiency testing and bridging studies.Journal of immunological methods · 2016Article
- Introduction to a Special Issue of the Journal of Immunological Methods: Building global resource programs to support HIV/AIDS clinical trial studies.Journal of immunological methods · 2014Article
- Managing Multi-center Flow Cytometry Data for Immune Monitoring.Cancer informatics · 2014Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The External Quality Assurance Program Oversight Laboratory (EQAPOL) Flow Cytometry Program assesses the proficiency of NIH/NIAID/DAIDS-supported and potentially other interested research laboratories in performing Intracellular Cytokine Staining (ICS) assays. The goal of the EQAPOL Flow Cytometry External Quality Assurance Program (EQAP) is to provide proficiency testing and remediation for participating sites. The program is not punitive; rather, EQAPOL aims to help sites identify areas for improvement. EQAPOL utilizes a highly standardized ICS assay to minimize variability and readily identify those sites experiencing technical difficulties with their assays. Here, we report the results of External Proficiency 3 (EP3) where participating sites performed a 7-color ICS assay. On average, sites perform well in the Flow Cytometry EQAP (median score is "Good"). The most common technical issues identified by the program involve protocol adherence and data analysis; these areas have been the focus of site remediation. The EQAPOL Flow Cytometry team is now in the process of expanding the program to 8-color ICS assays. Evaluating polyfunctional ICS responses would align the program with assays currently being performed in support of HIV immune monitoring assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.