ArticlePloS one2014
Comparison of the transmembrane mucins MUC1 and MUC16 in epithelial barrier function.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 117 citations in OpenAlex.
- Trial
- Muc16 contributes to protection against invasive pneumococcal infection originating from nasal colonization.Microbiology spectrum · 2026Article
- Serum CA125 is independently associated with adverse outcomes in primary Sjögren's syndrome-associated interstitial lung disease: a prospective multicenter cohort study.Respiratory research · 2026Observational
- Prior contact lens wear reduces susceptibility of the superficially-injured cornea to bacterial adhesion.PloS one · 2026Article
- Overlap of Genomic and Transcriptomic Genes Identified in Familial Eosinophilic Esophagitis.Gastroenterology · 2025Article
- Glycomics of cervicovaginal fluid from women at risk of preterm birth reveals immuno-regulatory epitopes that are hallmarks of cancer and viral glycosylation.Scientific reports · 2024Article
- MUC13 negatively regulates tight junction proteins and intestinal epithelial barrier integrity via protein kinase C.Journal of cell science · 2024Article
- Regional Conjunctival Differences in Glycocalyx Mucin Expression in Dry Eye and Normal Subjects.Investigative ophthalmology & visual science · 2024Article
- MUC1 and MUC16: critical for immune modulation in cancer therapeutics.Frontiers in immunology · 2024Review
- Role of Macrophage Colony-Stimulating Factor for Staphylococcal Infection in the Oral Cavity.Journal of clinical medicine · 2023Article
- Roles unveiled for membrane-associated mucins at the ocular surface using a Muc4 knockout mouse model.Scientific reports · 2023Article
- Sequencing Analysis ofCurrent issues in molecular biology · 2023Article
- Review
- MUC16 and TP53 family co-regulate tumor-stromal heterogeneity in pancreatic adenocarcinoma.Frontiers in oncology · 2023Article
- Luminal and Glandular Epithelial Cells from the Porcine Endometrium maintain Cell Type-Specific Marker Gene Expression in Air-Liquid Interface Culture.Stem cell reviews and reports · 2022Article
- A Mucin-Deficient Ocular Surface Mimetic Platform for Interrogating Drug Effects on Biolubrication, Antiadhesion Properties, and Barrier Functionality.ACS applied materials & interfaces · 2022Article
- Transmembrane Mucin 1 Blocks Fluorescein Ingress to Corneal Epithelium.Investigative ophthalmology & visual science · 2022Article
- Article
- Mucus and Mucins: The Underappreciated Host Defence System.Frontiers in cellular and infection microbiology · 2022Review
- Review
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Membrane-anchored mucins are present in the apical surface glycocalyx of mucosal epithelial cells, each mucosal epithelium having at least two of the mucins. The mucins have been ascribed barrier functions, but direct comparisons of their functions within the same epithelium have not been done. In an epithelial cell line that expresses the membrane-anchored mucins, MUC1 and MUC16, the mucins were independently and stably knocked down using shRNA. Barrier functions tested included dye penetrance, bacterial adherence and invasion, transepithelial resistance, tight junction formation, and apical surface size. Knockdown of MUC16 decreased all barrier functions tested, causing increased dye penetrance and bacterial invasion, decreased transepithelial resistance, surprisingly, disruption of tight junctions, and greater apical surface cell area. Knockdown of MUC1 did not decrease barrier function, in fact, barrier to dye penetrance and bacterial invasion increased significantly. These data suggest that barrier functions of membrane-anchored mucins vary in the context of other membrane mucins, and MUC16 provides a major barrier when present.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.