ArticlePloS one2014
Nanoscale protein diffusion by STED-based pair correlation analysis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Pair correlation microscopy of intracellular molecular transport.Nature protocols · 2025Review
- Article
- Super-resolution microscopy to study membrane nanodomains and transport mechanisms in the plasma membrane.Frontiers in molecular biosciences · 2024Review
- Review
- An EfficientInternational journal of molecular sciences · 2022Article
- Advanced Static and Dynamic Fluorescence Microscopy Techniques to Investigate Drug Delivery Systems.Pharmaceutics · 2021Review
- Back to the Future: Genetically Encoded Fluorescent Proteins as Inert Tracers of the Intracellular Environment.International journal of molecular sciences · 2020Review
- Dengue Virus Capsid Protein Dynamics Reveals Spatially Heterogeneous Motion in Live-Infected-Cells.Scientific reports · 2020Article
- z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics.Biophysical journal · 2020Article
- Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED-FCS.Nature protocols · 2019Article
- Label-free Multiscale Transport Imaging of the Living Cell.Biophysical journal · 2018Article
- Nanoscale Spatiotemporal Diffusion Modes Measured by Simultaneous Confocal and Stimulated Emission Depletion Nanoscopy Imaging.Nano letters · 2018Article
- Applications of STED fluorescence nanoscopy in unravelling nanoscale structure and dynamics of biological systems.Journal of biosciences · 2018Review
- Emerging views of the nucleus as a cellular mechanosensor.Nature cell biology · 2018Review
- Bridging the gap: Super-resolution microscopy of epithelial cell junctions.Tissue barriers · 2018Review
- Visualization of barriers and obstacles to molecular diffusion in live cells by spatial pair-cross-correlation in two dimensions.Biomedical optics express · 2018Article
- Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS.Nature communications · 2017Article
- Time-resolved biophysical approaches to nucleocytoplasmic transport.Computational and structural biotechnology journal · 2017Review
- Diffusion Tensor Analysis by Two-Dimensional Pair Correlation of Fluorescence Fluctuations in Cells.Biophysical journal · 2016Article
- Spatiotemporal Fluctuation Analysis: A Powerful Tool for the Future Nanoscopy of Molecular Processes.Biophysical journal · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe for the first time the combination between cross-pair correlation function analysis (pair correlation analysis or pCF) and stimulated emission depletion (STED) to obtain diffusion maps at spatial resolution below the optical diffraction limit (super-resolution). Our approach was tested in systems characterized by high and low signal to noise ratio, i.e. Capsid Like Particles (CLPs) bearing several (>100) active fluorescent proteins and monomeric fluorescent proteins transiently expressed in living Chinese Hamster Ovary cells, respectively. The latter system represents the usual condition encountered in living cell studies on fluorescent protein chimeras. Spatial resolution of STED-pCF was found to be about 110 nm, with a more than twofold improvement over conventional confocal acquisition. We successfully applied our method to highlight how the proximity to nuclear envelope affects the mobility features of proteins actively imported into the nucleus in living cells. Remarkably, STED-pCF unveiled the existence of local barriers to diffusion as well as the presence of a slow component at distances up to 500-700 nm from either sides of nuclear envelope. The mobility of this component is similar to that previously described for transport complexes. Remarkably, all these features were invisible in conventional confocal mode.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.