ArticleNature protocols2014
High-throughput microfluidic single-cell analysis pipeline for studies of signaling dynamics.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 121 citations in OpenAlex.
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- An outlook on the current challenges and opportunities in DNA data storage.Biotechnology advances · 2023Review
- High-throughput co-culture system for analysis of spatiotemporal cell-cell signaling.Biosensors & bioelectronics · 2023Article
- Discovery of New States of Immunomodulation for Vaccine Adjuvants via High Throughput Screening: Expanding Innate Responses to PRRs.ACS central science · 2023Article
- Revising immune cell coordination: Origins and importance of single-cell variation.European journal of immunology · 2022Review
- Multiplexed microfluidic chip for cell co-culture.The Analyst · 2022Article
- Spatiotemporal NF-κB dynamics encodes the position, amplitude, and duration of local immune inputs.Science advances · 2022Article
- NF-κB memory coordinates transcriptional responses to dynamic inflammatory stimuli.Cell reports · 2022Article
- Open-source personal pipetting robots with live-cell incubation and microscopy compatibility.Nature communications · 2022Article
- Review
- Computer vision reveals hidden variables underlying NF-κB activation in single cells.Science advances · 2021Article
- Assessing the influence of perfusion on cardiac microtissue maturation: A heart-on-chip platform embedding peristaltic pump capabilities.Biotechnology and bioengineering · 2021Article
- Building bridges between fields: bringing together development and homeostasis.Development (Cambridge, England) · 2021Review
- A design and optimization of a high throughput valve based microfluidic device for single cell compartmentalization and analysis.Scientific reports · 2021Article
- NF-κB responds to absolute differences in cytokine concentrations.Science signaling · 2021Article
- A Minireview of Microfluidic Scaffold Materials in Tissue Engineering.Frontiers in molecular biosciences · 2021Review
- Microfluidics in Single-Cell Virology: Technologies and Applications.Trends in biotechnology · 2020Review
- A Precise Microfluidic Assay in Single-Cell Profile for Screening of Transient Receptor Potential Channel Modulators.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Rapid lipolytic oscillations in ex vivo adipose tissue explants revealed through microfluidic droplet sampling at high temporal resolution.Lab on a chip · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Time-dependent analysis of dynamic processes in single live cells is a revolutionary technique for the quantitative studies of signaling networks. Here we describe an experimental pipeline and associated protocol that incorporate microfluidic cell culture, precise stimulation of cells with signaling molecules or drugs, live-cell microscopy, computerized cell tracking, on-chip staining of key proteins and subsequent retrieval of cells for high-throughput gene expression analysis using microfluidic quantitative PCR (qPCR). Compared with traditional culture dish approaches, this pipeline enhances experimental precision and throughput by orders of magnitude and introduces much-desired new capabilities in cell and fluid handling, thus representing a major step forward in dynamic single-cell analysis. A combination of microfluidic membrane valves, automation and a streamlined protocol now enables a single researcher to generate 1 million data points on single-cell protein localization within 1 week, in various cell types and densities, under 48 predesigned experimental conditions selected from different signaling molecules or drugs, their doses, timings and combinations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.