Evidence map›Paper›PMID 24966913›Full record

ArticleInternational journal of clinical and experimental pathology2014

Nogo-C contributes to HCC tumorigenesis via suppressing cell growth and its interactome analysis with comparative proteomics research.

Xing Liu, Shu-Jian Cui, Shi-Jun Zhu, De-Chun Geng, Long Yu

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Article in International journal of clinical and experimental pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

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0cells of the map it votes in
7citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Research advances on neurite outgrowth inhibitor B receptor.Journal of cellular and molecular medicine · 2020
    Review
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Xing LiuState Key Laboratory of Genetic Engineering, Fudan University Shanghai, China ; National Engineering Center for Biochip at Shanghai Shanghai, China.
Shu-Jian CuiCollege of Bioscience and Biotechnology, Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University Yangzhou, China.
Shi-Jun ZhuDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University Suzhou, China.
De-Chun GengDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University Suzhou, China.
Long YuState Key Laboratory of Genetic Engineering, Fudan University Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectsNeurite outgrowth inhibitor proteins (Nogos) comprise a family of three major members and are characterized by a conserved RHD domain. Among all the members, Nogo-B was identified to be significantly elevated and to play an important role in liver cirrhosis while Nogo-C was the shortest one and received little attention. The aim of this study is to investigate the relevance and mechanism of Nogo-C involved in Hepatocellular carcinoma (HCC).

methodsThe expression of Nogo-C in paired HCC specimens was measured with quantitative RT-PCR. The function of Nogo-C over expressing in SMMC-7721 and WRL-68 HCC cell lines were estimated through cell proliferation assay and colony formation assay. A proteome-wide identification of Nogo-C-binding proteins was performed using affinity purification combined with a highly sensitive mass spectrometric technique. The protein interactions were confirmed using co-IP and immunofluorescence confocal assays.

resultsCompared with the neighboring pathologically normal tissues, the expression of Nogo-C mRNA was extremely down-regulated in HCC specimens and was significantly related to greater tumor size and worse prognosis. Overexpression of Nogo-C in HCC cell lines resulted in an inhibition of cell growth. A total of 73 proteins were detected and considered in association with Nogo-C, among which B-raf and Nogo-B were validated.

conclusionWe identify Nogo-C as a tumor suppressor gene in HCC and B-raf as a novel interacting protein. These findings provide new directions for the mechanism research of Nogo family.

Indexed as

Cell ProliferationProtein Interaction MapsProteomicsAmino Acid SequenceCarcinoma, HepatocellularCell Line, TumorChromatography, AffinityChromatography, Reverse-PhaseDown-RegulationGene Expression Regulation, NeoplasticHumansImmunoprecipitationLiver NeoplasmsMolecular Sequence DataMyelin ProteinsNogo ProteinsBRAF protein, humanMyelin ProteinsNogo ProteinsProto-Oncogene Proteins B-rafRNA, MessengerRTN4 protein, humanB-rafcomparative proteomicsHCCinteractomeNogo-Ctumor suppressor gene

Identifiers

PMID24966913
PMCPMC4069910

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