ArticleJournal of immunology (Baltimore, Md. : 1950)2014
The majority of human memory B cells recognizing RhD and tetanus resides in IgM+ B cells.
Article in Journal of immunology (Baltimore, Md. : 1950), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Jo-1 autoantigen-specific B cells are skewed towards distinct functional B cell subsets in anti-synthetase syndrome patients.Arthritis research & therapy · 2021Article
- CD4+ T Cells Are Dispensable for Induction of Broad Heterologous HIV Neutralizing Antibodies in Rhesus Macaques.Frontiers in immunology · 2021Article
- Characterising the Phenotypic Diversity of Antigen-Specific Memory B Cells Before and After Vaccination.Frontiers in immunology · 2021Article
- Integrative transcriptome and chromatin landscape analysis reveals distinct epigenetic regulations in human memory B cells.Nature communications · 2020Article
- FcγR Binding and ADCC Activity of Human IgG Allotypes.Frontiers in immunology · 2020Article
- Highly Sensitive Flow Cytometric Detection of Residual B-Cells After Rituximab in Anti-Neutrophil Cytoplasmic Antibodies-Associated Vasculitis Patients.Frontiers in immunology · 2020Observational
- T-BetCell reports · 2018Article
- CD11c+ T-bet+ memory B cells: Immune maintenance during chronic infection and inflammation?Cellular immunology · 2017Review
- Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.Experimental and therapeutic medicine · 2017Article
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- Decoding the Human Immunoglobulin G-Glycan Repertoire Reveals a Spectrum of Fc-Receptor- and Complement-Mediated-Effector Activities.Frontiers in immunology · 2017Article
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- Optimisation ofWellcome open research · 2017Article
- Serological blind spots for variants of human IgG3 and IgG4 by a commonly used anti-immunoglobulin reagent.Transfusion · 2016Article
- Human memory B cells.Leukemia · 2016Review
- The Elements Steering Pathogenesis in IgG-Mediated Alloimmune Diseases.Journal of clinical immunology · 2016Review
- A Reassessment of IgM Memory Subsets in Humans.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Functional capacities of human IgM memory B cells in early inflammatory responses and secondary germinal center reactions.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Rapid Proliferation and Differentiation of a Subset of Circulating IgM Memory B Cells to a CpG/Cytokine Stimulus In Vitro.PloS one · 2015Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
B cell memory to T cell-dependent (TD) Ags are considered to largely reside in class-switched CD27(+) cells. However, we previously observed that anti-RhD (D) Igs cloned from two donors, hyperimmunized with D(+) erythrocytes, were predominantly of the IgM isotype. We therefore analyzed in this study the phenotype and frequency of D- and tetanus toxoid-specific B cells by culturing B cells in limiting dilution upon irradiated CD40L-expressing EL4.B5 cells and testing the culture supernatant. Most Ag-specific B cells for both TD Ags were found to reside in the IgM-expressing B cells, including CD27(-) B cells, in both hyperimmunized donors and nonhyperimmunized volunteers. Only shortly after immunization a sharp increase in Ag-specific CD27(+)IgG(+) B cells was observed. Next, B cells were enriched with D(+) erythrocyte ghosts and sorted as single cells. Sequencing of IGHV, IGLV, IGKV, and BCL6 genes from these D-specific B cell clones demonstrated that both CD27(-)IgM(+) and CD27(+)IgM(+) B cells harbored somatic mutations, documenting their Ag-selected nature. Furthermore, sequencing revealed a clonal relationship between the CD27(-)IgM(+), CD27(+)IgM(+), and CD27(+)IgG(+) B cell subsets. These data strongly support the recently described multiple layers of memory B cells to TD Ags in mice, where IgM(+) B cells represent a memory reservoir which can re-enter the germinal center and ensure replenishment of class-switched memory CD27(+) B cells from Ag-experienced precursors.
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