ArticleMolecular & cellular proteomics : MCP2014
Integrated transcriptomic and proteomic analysis of the bile stress response in a centenarian-originated probiotic Bifidobacterium longum BBMN68.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 83 citations in OpenAlex.
- The HUNT study identifies host genetic factors reproducibly associated with human gut microbiota composition.Nature genetics · 2026Article
- Probiogenomics as a Computational Biotechnology Framework: Safety-Gated Genome Analytics for Candidate Probiotic Prioritization and Validation.Computational and structural biotechnology journal · 2026Review
- Engineered probiotics for inflammatory bowel disease therapy: mechanisms, delivery strategies, and precision medicine.Frontiers in microbiology · 2025Review
- Next-generation probiotics and engineered BEVs for precision therapeutics in osteoporosis.Frontiers in nutrition · 2025Review
- Next-Generation Probiotics and Chronic Diseases: A Review of Current Research and Future Directions.Journal of agricultural and food chemistry · 2024Review
- Review
- CsbD, a Novel Group B Streptococcal Stress Response Factor That Contributes to Bacterial Resistance against Environmental Bile Salts.Journal of bacteriology · 2023Article
- Novel Insights into the Molecular Mechanisms Underlying Robustness and Stability in Probiotic Bifidobacteria.Applied and environmental microbiology · 2023Article
- The Pleiotropic Effects of Carbohydrate-Mediated Growth Rate Modifications inMicroorganisms · 2023Article
- Stress Response in Bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2022Review
- Pan-genome evolution and its association with divergence of metabolic functions in Bifidobacterium genus.World journal of microbiology & biotechnology · 2022Article
- Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria.Applied and environmental microbiology · 2022Article
- Evaluation of Selected Bacteria and Yeast for Probiotic Potential in Poultry Production.Microorganisms · 2022Article
- Transcriptome analysis revealed growth phase-associated changes of a centenarian-originated probiotic Bifidobacterium animalis subsp. lactis A6.BMC microbiology · 2022Article
- Comparative Genomic Analysis Determines the Functional Genes Related to Bile Salt Resistance inMicroorganisms · 2021Article
- Dose-Dependent Beneficial Effects of Tryptophan and Its Derived Metabolites onMicroorganisms · 2021Article
- Exoproteome Perspective on the Bile Stress Response ofProteomes · 2021Article
- Adhesion mechanisms ofBioscience of microbiota, food and health · 2021Article
- The genus bifidobacterium: From genomics to functionality of an important component of the mammalian gut microbiota running title: Bifidobacterial adaptation to and interaction with the host.Computational and structural biotechnology journal · 2021Review
- Tools to map target genes of bacterial two-component system response regulators.Environmental microbiology reports · 2020Review
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bifidobacteria are natural inhabitants of the human gastrointestinal tract and well known for their health-promoting effects. Tolerance to bile stress is crucial for bifidobacteria to survive in the colon and to exert their beneficial actions. In this work, RNA-Seq transcriptomic analysis complemented with proteomic analysis was used to investigate the cellular response to bile in Bifidobacterium longum BBMN68. The transcript levels of 236 genes were significantly changed (≥ threefold, p < 0.001) and 44 proteins were differentially abundant (≥1.6-fold, p < 0.01) in B. longum BBMN68 when exposed to 0.75 g l(-1) ox-bile. The hemolysin-like protein and bile efflux systems were significantly over produced, which might prevent bile adsorption and exclude bile, respectively. The cell membrane composition was modified probably by an increase of cyclopropane fatty acid and a decrease of transmembrane proteins, resulting in a cell membrane more impermeable to bile salts. Our hypothesis was later confirmed by surface hydrophobicity assay. The transcription of genes related to xylose utilization and bifid shunt were up-regulated, which increased the production of ATP and reducing equivalents to cope with bile-induced damages in a xylan-rich colon environment. Bile salts signal the B. longum BBMN68 to gut entrance and enhance the expression of esterase and sortase associated with adhesion and colonization in intestinal tract, which was supported by a fivefold increased adhesion ability to HT-29 cells by BBMN68 upon bile exposure. Notably, bacterial one-hybrid and EMSA assay revealed that the two-component system senX3-regX3 controlled the expression of pstS in bifidobacteria and the role of this target gene in bile resistance was further verified by heterologous expression in Lactococcus lactis. Taken altogether, this study established a model for global response mechanisms in B. longum to bile.
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