ArticleJournal of experimental botany2014
Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 54 citations in OpenAlex.
- Functional and structural insights into cyanobacterial COThe Plant journal : for cell and molecular biology · 2026Review
- Functional characterization of bicarbonate transporters from the cyanobacterial SbtA2 family and subsequent expression in tobacco.Journal of experimental botany · 2026Article
- Structure of Chlamydomonas reinhardtii LciA guided the engineering of FNT family proteins to gain bicarbonate transport activity.Nature plants · 2026Article
- Understanding carboxysomes to enhance carbon fixation in crops.Biochemical Society transactions · 2025Review
- Engineering the cyanobacterial ATP-driven BCT1 bicarbonate transporter for functional targeting to C3 plant chloroplasts.Journal of experimental botany · 2024Article
- Molecular mechanism underlying transport and allosteric inhibition of bicarbonate transporter SbtA.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- New discoveries expand possibilities for carboxysome engineering.Current opinion in microbiology · 2021Review
- Engineered Accumulation of Bicarbonate in Plant Chloroplasts: Known Knowns and Known Unknowns.Frontiers in plant science · 2021Review
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- Prospects for Engineering Biophysical COAnnual review of plant biology · 2020Review
- Photosynthesis research: a model to bridge fundamental science, translational products, and socio-economic considerations in agriculture.Journal of experimental botany · 2020Review
- Installation of authentic BicA and SbtA proteins to the chloroplast envelope membrane is achieved by the proteolytic cleavage of chimeric proteins in Arabidopsis.Scientific reports · 2020Article
- Recent advances in understanding and improving photosynthesis.Faculty reviews · 2020Review
- Elucidation and genetic intervention of COJournal of biosciences · 2020Article
- Structural mechanism of the active bicarbonate transporter from cyanobacteria.Nature plants · 2019Article
- Physiological performance of transplastomic tobacco plants overexpressing aquaporin AQP1 in chloroplast membranes.Journal of experimental botany · 2018Article
- Setting sub-organellar sights: accurate targeting of multi-transmembrane-domain proteins to specific chloroplast membranes.Journal of experimental botany · 2017Article
- Can increased leaf photosynthesis be converted into higher crop mass production? A simulation study for rice using the crop model GECROS.Journal of experimental botany · 2017Article
- Engineering photosynthesis: progress and perspectives.F1000Research · 2017Review
- Biochemical and synthetic biology approaches to improve photosynthetic COCurrent opinion in chemical biology · 2016Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improving global yields of agricultural crops is a complex challenge with evidence indicating benefits in productivity are achieved by enhancing photosynthetic carbon assimilation. Towards improving rates of CO2 capture within leaf chloroplasts, this study shows the versatility of plastome transformation for expressing the Synechococcus PCC7002 BicA bicarbonate transporter within tobacco plastids. Fractionation of chloroplast membranes from transplastomic tob(BicA) lines showed that ~75% of the BicA localized to the thylakoid membranes and ~25% to the chloroplast envelope. BicA levels were highest in young emerging tob(BicA) leaves (0.12 μmol m(-2), ≈7mg m(-2)) accounting for ~0.1% (w/w) of the leaf protein. In these leaves, the molar amount of BicA was 16-fold lower than the abundant thylakoid photosystem II D1 protein (~1.9 μmol m(-2)) which was comparable to the 9:1 molar ratio of D1:BicA measured in air-grown Synechococcus PCC7002 cells. The BicA produced had no discernible effect on chloroplast ultrastructure, photosynthetic CO2-assimilation rates, carbon isotope discrimination, or growth of the tob(BicA) plants, implying that the bicarbonate transporter had little or no activity. These findings demonstrate the utility of plastome transformation for targeting bicarbonate transporter proteins into the chloroplast membranes without impeding growth or plastid ultrastructure. This study establishes the span of experimental measurements required to verify heterologous bicarbonate transporter function and location in chloroplasts and underscores the need for more detailed understanding of BicA structure and function to identify solutions for enabling its activation and operation in leaf chloroplasts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.