ArticleJournal of virology2014
Development of giant bacteriophage ϕKZ is independent of the host transcription apparatus.
Article in Journal of virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
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Who cites it
125 citing papers in PubMed.
- Phage anti-defense genes targeting NADVirulence · 2026Article
- Characterization and genomic analysis of a jumbo Aeromonas hydrophila phage BMBAH1, representing a candidate novel viral genus.Virus research · 2026Article
- Phage host range: determinants, dynamics and applications.Nature reviews. Microbiology · 2026Review
- Article
- The biology of jumbo phages.Nature communications · 2026Review
- Rise of the viral giants: common themes underlying genome gigantism in eukaryotic viruses and bacteriophages.Current opinion in microbiology · 2026Review
- The phage nucleus synergizes with an anti-defense protein to resist bacterial immunity.Cell reports · 2026Article
- Molecular basis for anti-jumbo phage immunity by AVAST type 5.Molecular cell · 2026Article
- Structure of the bacteriophage PhiKZ non-virion RNA polymerase bound to a p119L open promoter analogue.IUCrJ · 2026Article
- Isolation, Characterization, and Evaluation of a Lytic Jumbo Phage Z90 AgainstAntibiotics (Basel, Switzerland) · 2025Article
- Bacteriophage genome-wide transposon mutagenesis.bioRxiv : the preprint server for biology · 2025Article
- The complex developmental mechanisms of nucleus-forming jumbo phages.Current opinion in microbiology · 2025Review
- A novel lineage of large aquatic bacteriophages identified through metagenomics.Archives of virology · 2025Article
- The Biology of Nucleus-Forming Jumbo Phages.Annual review of genetics · 2025Review
- Article
- Two novel polyvalent phages: a promising approach for cross-order pathogen control in aquaculture.Virology journal · 2025Article
- The Current Landscape of Phage-Antibiotic Synergistic (PAS) Interactions.Antibiotics (Basel, Switzerland) · 2025Review
- Bacteriophage-driven microbial phenotypic heterogeneity: ecological and biogeochemical importance.NPJ biofilms and microbiomes · 2025Review
- Article
- Sequential membrane- and protein-bound organelles compartmentalize genomes during phage infection.Cell host & microbe · 2025Article
65 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
Funding
Abstract
unlabelledPseudomonas aeruginosa bacteriophage ϕKZ is the type representative of the giant phage genus, which is characterized by unusually large virions and genomes. By unraveling the transcriptional map of the ∼ 280-kb ϕKZ genome to single-nucleotide resolution, we combine 369 ϕKZ genes into 134 operons. Early transcription is initiated from highly conserved AT-rich promoters distributed across the ϕKZ genome and located on the same strand of the genome. Early transcription does not require phage or host protein synthesis. Transcription of middle and late genes is dependent on protein synthesis and mediated by poorly conserved middle and late promoters. Unique to ϕKZ is its ability to complete its infection in the absence of bacterial RNA polymerase (RNAP) enzyme activity. We propose that transcription of the ϕKZ genome is performed by the consecutive action of two ϕKZ-encoded, noncanonical multisubunit RNAPs, one of which is packed within the virion, another being the product of early genes. This unique, rifampin-resistant transcriptional machinery is conserved within the diverse giant phage genus. IMPORTANCE: The data presented in this paper offer, for the first time, insight into the complex transcriptional scheme of giant bacteriophages. We show that Pseudomonas aeruginosa giant phage ϕKZ is able to infect and lyse its host cell and produce phage progeny in the absence of functional bacterial transcriptional machinery. This unique property can be attributed to two phage-encoded putative RNAP enzymes, which contain very distant homologues of bacterial β and β'-like RNAP subunits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.