ArticlePlant physiology2014
Genetic Evidence That Chain Length and Branch Point Distributions Are Linked Determinants of Starch Granule Formation in Arabidopsis.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 57 citations in OpenAlex.
- Understanding and modifying starch metabolism to limit yield losses in field-grown cassava.Plant physiology · 2026Article
- Starch Synthase 3 isoforms are essential for normal starch granule initiation in wheat endosperm.The New phytologist · 2026Article
- A MYB61-SWB9-KOs module regulates grain chalkiness via gibberellin biosynthesis in rice endosperm.Plant biotechnology journal · 2025Article
- Food as Medicine: Curbing Type-2 Diabetes Prevalence Through Consumption of High Amylose Starchy Foods in Sub-Saharan Africa.Advanced pharmaceutical bulletin · 2025Review
- Covalently linked phosphate monoesters on alpha-polyglucans reduce substrate affinity of branching enzymes.Carbohydrate polymers · 2025Article
- A point mutation in the photosystem II protein PsbW disrupts thylakoid organization and alters starch granule formation.Plant physiology · 2025Article
- Transcriptome and Metabolome Analyses Reveal the Regulatory Mechanism ofPlants (Basel, Switzerland) · 2024Article
- Modulation of the Arabidopsis Starch Metabolic Network by the Cytosolic Acetyl-CoA Pathway in the Context of the Diurnal Illumination Cycle.International journal of molecular sciences · 2024Article
- Effects of the Molecular Structure of Starch in Foods on Human Health.Foods (Basel, Switzerland) · 2023Review
- LIKE EARLY STARVATION 1 and EARLY STARVATION 1 promote and stabilize amylopectin phase transition in starch biosynthesis.Science advances · 2023Article
- Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity.BMC biology · 2022Article
- Starch branching enzymes as putative determinants of postharvest quality in horticultural crops.BMC plant biology · 2021Review
- Gene Coexpression Network Analysis Indicates that Hub Genes Related to Photosynthesis and Starch Synthesis Modulate Salt Stress Tolerance inInternational journal of molecular sciences · 2021Article
- A review of starch biosynthesis in cereal crops and its potential breeding applications in rice (PeerJ · 2021Article
- Starch and Glycogen Analyses: Methods and Techniques.Biomolecules · 2020Review
- Theoretical and experimental approaches to understand the biosynthesis of starch granules in a physiological context.Photosynthesis research · 2020Article
- Two Plastidial Coiled-Coil Proteins Are Essential for Normal Starch Granule Initiation in Arabidopsis.The Plant cell · 2018Article
- Bioinformatic andFrontiers in plant science · 2018Article
- Distinct Functions of STARCH SYNTHASE 4 Domains in Starch Granule Formation.Plant physiology · 2018Article
- Design starch: stochastic modeling of starch granule biogenesis.Biochemical Society transactions · 2017Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The major component of starch is the branched glucan amylopectin. Structural features of amylopectin, such as the branching pattern and the chain length distribution, are thought to be key factors that enable it to form semicrystalline starch granules. We varied both structural parameters by creating Arabidopsis (Arabidopsis thaliana) mutants lacking combinations of starch synthases (SSs) SS1, SS2, and SS3 (to vary chain lengths) and the debranching enzyme ISOAMYLASE1-ISOAMYLASE2 (ISA; to alter branching pattern). The isa mutant accumulates primarily phytoglycogen in leaf mesophyll cells, with only small amounts of starch in other cell types (epidermis and bundle sheath cells). This balance can be significantly shifted by mutating different SSs. Mutation of SS1 promoted starch synthesis, restoring granules in mesophyll cell plastids. Mutation of SS2 decreased starch synthesis, abolishing granules in epidermal and bundle sheath cells. Thus, the types of SSs present affect the crystallinity and thus the solubility of the glucans made, compensating for or compounding the effects of an aberrant branching pattern. Interestingly, ss2 mutant plants contained small amounts of phytoglycogen in addition to aberrant starch. Likewise, ss2ss3 plants contained phytoglycogen, but were almost devoid of glucan despite retaining other SS isoforms. Surprisingly, glucan production was restored in the ss2ss3isa triple mutants, indicating that SS activity in ss2ss3 per se is not limiting but that the isoamylase suppresses glucan accumulation. We conclude that loss of only SSs can cause phytoglycogen production. This is readily degraded by isoamylase and other enzymes so it does not accumulate and was previously unnoticed.
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Registered trials
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