ArticlePloS one2014
FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- A single nucleotide mutation ofHorticulture research · 2026Article
- Genome-wide identification and comprehensive analysis of the FtsH gene family in wheat.Molecular biology reports · 2025Article
- A Genome-Wide Characterization of Receptor-like Cytoplasmic Kinase IV Subfamily Members inPlants (Basel, Switzerland) · 2024Article
- Arabidopsis TIC236 contributes to proplastid development and chloroplast biogenesis during embryogenesis.Frontiers in plant science · 2024Article
- Chloroplast import motor subunits FtsHi1 and FtsHi2 are located on opposite sides of the inner envelope membrane.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Differential Expression of miRNAs Involved in Response toPlants (Basel, Switzerland) · 2023Article
- Genetic mapping and molecular characterization of the delayed green geneFrontiers in plant science · 2023Article
- Recent Advances in Understanding the Structural and Functional Evolution of FtsH Proteases.Frontiers in plant science · 2022Review
- The FtsHi Enzymes ofInternational journal of molecular sciences · 2021Review
- Abundance of metalloprotease FtsH12 modulates chloroplast development in Arabidopsis thaliana.Journal of experimental botany · 2021Article
- The Plastid-Localized AtFtsHi3 Pseudo-Protease ofFrontiers in plant science · 2021Article
- Plastid-Localized EMB2726 Is Involved in Chloroplast Biogenesis and Early Embryo Development inFrontiers in plant science · 2021Article
- The FtsH-Inactive Protein FtsHi5 Is Required for Chloroplast Development and Protein Accumulation in Chloroplasts at Low Ambient Temperature in Arabidopsis.Frontiers in plant science · 2021Article
- Unraveling Hidden Components of the Chloroplast Envelope Proteome: Opportunities and Limits of Better MS Sensitivity.Molecular & cellular proteomics : MCP · 2019Article
- Reduced expression of the proteolytically inactive FtsH members has impacts on the Darwinian fitness of Arabidopsis thaliana.Journal of experimental botany · 2019Article
- The Chloroplast Envelope Protease FTSH11 - Interaction With CPN60 and Identification of Potential Substrates.Frontiers in plant science · 2019Article
- A Ycf2-FtsHi Heteromeric AAA-ATPase Complex Is Required for Chloroplast Protein Import.The Plant cell · 2018Article
- Plastidial NAD-Dependent Malate Dehydrogenase: A Moonlighting Protein Involved in Early Chloroplast Development through Its Interaction with an FtsH12-FtsHi Protease Complex.The Plant cell · 2018Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplast formation is associated with embryo development and seedling growth. However, the relationship between chloroplast differentiation and embryo development remains unclear. Five FtsHi genes that encode proteins with high similarity to FtsH proteins, but lack Zn2+-binding motifs, are present in the Arabidopsis genome. In this study, we showed that T-DNA insertion mutations in the Arabidopsis FtsHi4 gene resulted in embryo arrest at the globular-to-heart-shaped transition stage. Transmission electron microscopic analyses revealed abnormal plastid differentiation with a severe defect in thylakoid formation in the mutant embryos. Immunocytological studies demonstrated that FtsHi4 localized in chloroplasts as a thylakoid membrane-associated protein, supporting its essential role in thylakoid membrane formation. We further showed that FtsHi4 forms protein complexes, and that there was a significant reduction in the accumulation of D2 and PsbO (two photosystem II proteins) in mutant ovules. The role of FtsHi4 in chloroplast development was confirmed using an RNA-interfering approach. Additionally, mutations in other FtsHi genes including FtsHi1, FtsHi2, and FtsHi5 caused phenotypic abnormalities similar to ftshi4 with respect to plastid differentiation during embryogenesis. Taken together, our data suggest that FtsHi4, together with FtsHi1, FtsHi2, and FtsHi5 are essential for chloroplast development in Arabidopsis.
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